葡萄糖的酶动力学测定（作者译）
[Enzyme kinetic determination of glucose (author's transl)].
作者信息
Keller H, Wolf V
出版信息
J Clin Chem Clin Biochem. 1976 Jan;14(1):27-30.
PMID:180230
Abstract
A reaction kinetic method is described for the determination of glucose in biological fluids. Glucose is oxidized to gluconic acid and NAD is reduced to NADH in the presence of glucose dehydrogenase. The rate of formation of NADH is followed in a LKB Reaction-Rate-Meter. Together with the analogue read-out, the rate of reaction is determined with a newly developed kinetic calculator. The specifications of the calculator are given. The new method has the advantage of a high rate of throughput and good reproducibility.
摘要
本文描述了一种用于测定生物流体中葡萄糖的反应动力学方法。在葡萄糖脱氢酶存在的情况下，葡萄糖被氧化为葡萄糖酸，NAD被还原为NADH。在LKB反应速率计中跟踪NADH的形成速率。结合模拟读数，使用新开发的动力学计算器确定反应速率。给出了该计算器的规格。新方法具有高通量和良好重现性的优点。
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