School of Pharmacy & School of Biological and Food Engineering, National-Local Joint Engineering Research Center of Biomass Refining and High-Quality Utilization, Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, Changzhou University, Changzhou 213164, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.
Bioresour Technol. 2023 Dec;389:129819. doi: 10.1016/j.biortech.2023.129819. Epub 2023 Oct 4.
2,5-Dihydroxymethylfuran and furfuryl alcohol serve as versatile building-blocks in pharmaceuticals, polymers, and value-added intermediates. To develop an efficient and sustainable method for their production from biomass, a combined approach using deep eutectic solvent Citric acid:Betaine (CTA:BT) for bagasse catalysis and recombinant E. coli SCFD23 for bioreduction of bagasse-derived 5-hydroxymethylfurfural and furfural was devised. Bagasse was effectively transformed into 5-hydroxymethylfurfural (48 mM) and furfural (14 mM) in CTA:BT (8 wt%)-water at 170 °C for 30 min. Bioreduction of 5-hydroxymethylfurfural and furfural by SCFD23 cell co-expressing formate dehydrogenase and NAD(P)H-dependent aldehyde reductase (SsCR) yielded 2,5-dihydroxymethylfuran (90.0 % yield) and furfuryl alcohol (99.0 % yield) in 6 h, using biomass-derived formic acid, xylose and glucose as co-substrates. Molecular docking confirmed the stable binding and reductase activity of SsCR with the biomass-derived 5-hydroxymethylfurfural and furfural. An efficient and eco-friendly chemobiological approach was applied for co-production of 2,5-dihydroxymethylfuran and furfuryl alcohol from biomass in one-pot two-step reaction.
2,5-二羟甲基呋喃和糠醇可用作药物、聚合物和增值中间体的多功能构建块。为了从生物质中开发出高效、可持续的生产方法，设计了一种使用深共晶溶剂柠檬酸：甜菜碱（CTA:BT）催化蔗渣和重组大肠杆菌 SCFD23 生物还原蔗渣衍生的 5-羟甲基糠醛和糠醛的组合方法。在 170°C 下，在 8wt%CTA:BT-水中反应 30 分钟，蔗渣可有效地转化为 5-羟甲基糠醛（48mM）和糠醛（14mM）。SCFD23 细胞共表达甲酸脱氢酶和 NAD(P)H 依赖型醛还原酶（SsCR）进行 5-羟甲基糠醛和糠醛的生物还原，使用生物质衍生的甲酸、木糖和葡萄糖作为共底物，可得到 2,5-二羟甲基呋喃（90.0%收率）和糠醇（99.0%收率）。分子对接证实了 SsCR 与生物质衍生的 5-羟甲基糠醛和糠醛的稳定结合和还原酶活性。应用一种高效、环保的化学生物学方法，在一锅两步反应中从生物质中同时生产 2,5-二羟甲基呋喃和糠醇。
