嗜黏蛋白阿克曼氏菌及其细胞外囊泡对小鼠小肠中血清素能基因表达的调节作用。

The impact of Akkermansia muciniphila and its extracellular vesicles in the regulation of serotonergic gene expression in a small intestine of mice.

作者信息

Yaghoubfar Rezvan, Zare BanadKoki Ehsan, Ashrafian Fatemeh, Shahryari Arefeh, Kariman Arian, Davari Mehdi, Fateh Abolfazl, Khatami Shohreh, Siadat Seyed Davar

机构信息

Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.

Department of Microbiology, Shahed University, Tehran, Iran.

出版信息

Anaerobe. 2023 Oct;83:102786. doi: 10.1016/j.anaerobe.2023.102786. Epub 2023 Oct 4.

DOI:10.1016/j.anaerobe.2023.102786

PMID:37797929

Abstract

OBJECTIVES

A better understanding of host-microbe interactions as a cross-talk between the gastrointestinal (GI) tract and the gut microbiota can help treat and prevent GI disorders by improving the maintenance of GI homeostasis. The gut microbiota can affect signaling molecules, such as serotonin, which regulates endocrine systems through the GI tract. Moreover, studying the effects of gut microbiota in the small intestine on the human GI tract health is pivotal.

METHODS

Male C57BL/6J mice (n = 30, 10 mice per group) were orally gavaged with 200 μL of PBS (control group); mice in group II were orally gavaged with 109 colony-forming units (CFU)/200 μL of viable A. muciniphila, suspended in PBS (A. muciniphila group); and mice in group III were orally gavaged with 10 μg of protein/200 μL of EVs (A. muciniphila-EV group) once daily for four weeks. The gene expression of serotonin system-related genes (Slc6a4, Tph1, Mao, Htr3, Htr4, and Htr7) was examined by quantitative real-time PCR (qPCR) method.

RESULTS

Based on the results, A. muciniphila significantly affected the mRNA expression of genes related to the serotonin system (Tph1, Mao, Htr3B, and Htr7) in the duodenum and (Htr3B, Htr4 and Htr7) in the ileum of mice (P < 0.05). Moreover, A. muciniphila-derived EVs affected the expression of major genes related to the serotonin system (Tph1, slc6a4a, Mao, Htr3B, Htr4, and Htr7) in the duodenum and ileum of mice (P < 0.05).

CONCLUSIONS

The present findings may pave the way for further investigation of the effects of strain-specific probiotics on the serotonergic system, which is currently in its infancy.

摘要

目的

更好地理解宿主与微生物的相互作用，即胃肠道与肠道微生物群之间的相互交流，有助于通过改善胃肠道内环境稳定的维持来治疗和预防胃肠道疾病。肠道微生物群可影响信号分子，如血清素，血清素通过胃肠道调节内分泌系统。此外，研究小肠中肠道微生物群对人类胃肠道健康的影响至关重要。

方法

将雄性C57BL/6J小鼠（n = 30，每组10只小鼠）经口灌胃200 μL PBS（对照组）；第二组小鼠经口灌胃10⁹ 菌落形成单位（CFU）/200 μL 活的嗜黏蛋白阿克曼菌，悬浮于PBS中（嗜黏蛋白阿克曼菌组）；第三组小鼠经口灌胃10 μg蛋白质/200 μL 细胞外囊泡（嗜黏蛋白阿克曼菌-细胞外囊泡组），每天一次，持续四周。采用定量实时PCR（qPCR）方法检测血清素系统相关基因（Slc6a4、Tph1、Mao、Htr3、Htr4和Htr7）的基因表达。

结果

基于结果，嗜黏蛋白阿克曼菌显著影响小鼠十二指肠中血清素系统相关基因（Tph¹、Mao、Htr3B和Htr7）以及回肠中（Htr3B、Htr4和Htr7）的mRNA表达（P < 0.05）。此外，嗜黏蛋白阿克曼菌来源的细胞外囊泡影响小鼠十二指肠和回肠中血清素系统主要相关基因（Tph1、slc6a4a、Mao、Htr3B、Htr4和Htr7）的表达（P < 0.05）。