Whyte A, Ockleford C D, Wooding F B, Hamon M, Allen W R, Kellie S
Cell Tissue Res. 1986;246(2):343-52. doi: 10.1007/BF00215896.
The establishment of a monolayer culture of cells derived from the girdle region of a 34-day-old donkey conceptus is described. These cells have had over 100 repeated passages in culture. Low levels of pregnant mares' serum gonadotrophin (PMSG, eCG) could be detected in the cells by indirect immunofluorescence using some monoclonal anti-eCG antibodies, but the cells did not secrete eCG as measured by radioimmunoassay or inhibition of haemagglutination. There was marked nuclear polymorphism with binucleate and occasional multinucleate cells. The cells were strongly reactive with wheatgerm agglutinin and concanavalin A suggesting the synthesis of many glycosylated products. Some cells were reactive with antisera to prekeratin, others with antisera to vimentin. The cells also contained actin (showing peculiar intercellular communications), alpha-actinin and tubulin. They were able to metabolize certain steroid precursors, but there was no definitive evidence for the presence of aromatase or delta 5-3 beta-hydroxysteroid dehydrogenase in these cells. This cell line appears to resemble trophectodermal girdle epithelium at a stage of development prior to the onset of eCG production, and may be useful in studies on the control of expression of this substance.
本文描述了从34日龄驴胚胎带区衍生的细胞单层培养物的建立。这些细胞在培养中已传代100多次。使用一些单克隆抗eCG抗体通过间接免疫荧光法可在细胞中检测到低水平的孕马血清促性腺激素（PMSG，eCG），但通过放射免疫测定或血凝抑制法测量，这些细胞不分泌eCG。存在明显的核多态性，有双核细胞和偶尔的多核细胞。这些细胞与麦胚凝集素和伴刀豆球蛋白A强烈反应，表明合成了许多糖基化产物。一些细胞与抗前角蛋白抗血清反应，另一些与抗波形蛋白抗血清反应。这些细胞还含有肌动蛋白（显示出特殊的细胞间通讯）α-辅肌动蛋白和微管蛋白。它们能够代谢某些类固醇前体，但没有确凿证据表明这些细胞中存在芳香化酶或δ5-3β-羟基类固醇脱氢酶。该细胞系在eCG产生开始之前的发育阶段似乎类似于滋养外胚层带上皮，可能有助于研究该物质表达的调控。
