Faculty of Veterinary Medicine, Okayama University of Science, Ikoino-oka 1-3, Imabari Ehime, Japan.
Sci Rep. 2023 Oct 5;13(1):16823. doi: 10.1038/s41598-023-44062-1.
Canine pulmonary adenocarcinoma (PAC) resembles human lung tumors in never-smokers, but it is rarer than human pulmonary adenocarcinoma. Therefore, research on canine PAC is challenging. In the present study, we successfully established various novel canine PAC cell lines from a single lesion in a dog, including two parent cell lines and fourteen cloned cell lines, and characterized their cellular properties in vitro. Several of these cell lines showed epithelial-mesenchymal transition (EMT)-like and/or cancer stem cell (CSCs)-like phenotypes. We additionally assessed the sensitivity of the cells to vinorelbine in vitro. Three clonal lines, two of which showed EMT- and CSC-like phenotypes, were resistant to vinorelbine. Furthermore, we evaluated the expression and activation status of EGFR, HER2, and Ras signaling factors. The findings indicated that the cell lines we established preserved the expression and activation of these factors to varying extents. These novel canine PAC cell lines can be utilized in future research for understanding the pathogenesis and development of treatments for canine PAC.
犬肺腺癌 (PAC) 在从不吸烟的人群中类似于人类的肺肿瘤，但比人类肺腺癌更为罕见。因此，犬 PAC 的研究具有挑战性。在本研究中，我们成功地从一只狗的单个病变中建立了各种新型的犬 PAC 细胞系，包括两个亲本细胞系和十四个克隆细胞系，并在体外对其细胞特性进行了表征。其中一些细胞系表现出上皮-间充质转化 (EMT)-样和/或癌症干细胞 (CSC)-样表型。我们还评估了这些细胞对长春瑞滨的体外敏感性。三个克隆系，其中两个表现出 EMT 和 CSC 样表型，对长春瑞滨具有耐药性。此外，我们评估了 EGFR、HER2 和 Ras 信号因子的表达和激活状态。研究结果表明，我们建立的细胞系在不同程度上保留了这些因子的表达和激活。这些新型犬 PAC 细胞系可用于未来的研究，以了解犬 PAC 的发病机制和治疗方法的开发。
