Aimar C, Vilain C, Delarue M, Grant N
Cell Differ. 1986 Dec;19(4):245-52. doi: 10.1016/0045-6039(86)90101-6.
The effects of amphibian egg cytoplasm extracted at different times after activation and during the first four cleavages on cytokinesis were examined. Extracts of artificially activated or fertilized Xenopus or Pleurodeles eggs taken at the time of activation (T = 0) provoked precocious cleavage furrows in Pleurodeles eggs. Between T = 0.25 and T = 0.75 of the first cell cycle, the period corresponding to interphase, an inhibitory effect was found, and the division of injected eggs was delayed up to 30%. After T = 0.75, that is during mitosis, the cleavage induction effect was observed again. These enhancing and inhibitory effects were also found in the two fractions obtained following gel filtration of the cytoplasmic extracts. These experiments support the hypothesis that two antagonistic factors control cytokinesis. The inhibitory factor is active only during interphase, while the positive factor is present during mitosis and appears to regulate cytokinesis.
研究了在激活后不同时间以及前四次卵裂期间提取的两栖类卵细胞胞质对胞质分裂的影响。在激活时（T = 0）取自人工激活或受精的非洲爪蟾或有肋蝾螈卵的提取物，可在有肋蝾螈卵中引发早熟的分裂沟。在第一个细胞周期的T = 0.25至T = 0.75之间，即对应于间期的时期，发现有抑制作用，注射卵的分裂延迟达30%。在T = 0.75之后，即在有丝分裂期间，再次观察到分裂诱导作用。在对胞质提取物进行凝胶过滤后得到的两个组分中也发现了这些增强和抑制作用。这些实验支持了这样一种假说，即两种拮抗因子控制着胞质分裂。抑制因子仅在间期有活性，而阳性因子在有丝分裂期间存在，并且似乎调节胞质分裂。
