Aimar C, Vilain C, Delarue M
Cell Differ. 1983 Dec;13(4):293-300. doi: 10.1016/0045-6039(83)90039-8.
Injections of extracts of Xenopus egg cytoplasm induce precocious cleavage furrows in activated Pleurodeles eggs. This cleavage timing system (CTS) activity is retained in two distinct fractions separated by gel filtration. Acceleration of the first cell cycle of the Pleurodeles egg is directly related to the concentration of the injected extracts. However, the maximum advance of the cleavage cycle does not exceed 25 to 30% of the control cycle. Preliminary characterization of these fractions indicates that the CTS effect is abolished after protease but not RNAse treatment, and that the CTS factors are thermostable. The phase of the egg cell cycle sensitive to the CTS factors, which act as a 'clock system mechanism', is discussed.
注射非洲爪蟾卵细胞质提取物可诱导激活后的美西螈卵过早出现卵裂沟。这种卵裂定时系统（CTS）活性保留在通过凝胶过滤分离出的两个不同组分中。美西螈卵第一个细胞周期的加速与注射提取物的浓度直接相关。然而，卵裂周期的最大提前量不超过对照周期的25%至30%。对这些组分的初步表征表明，蛋白酶处理后CTS效应消失，但RNA酶处理后不会消失，并且CTS因子具有热稳定性。本文讨论了卵细胞周期中对作为“时钟系统机制”起作用的CTS因子敏感的阶段。
