Department of Gastroenterology, Faculty of Medicine, Manisa Celal Bayar University, Manisa, Turkey.
Department of Biology, Faculty of Arts and Sciences, Manisa Celal Bayar University, Manisa, Turkey.
BMC Gastroenterol. 2023 Oct 5;23(1):345. doi: 10.1186/s12876-023-02985-z.
Ulcerative colitis (UC) is a chronic inflammatory bowel disease that develops due to the impaired immune response in genetically susceptible individuals, and its etiopathogenesis is not fully elucidated. IL-17 A is a cytokine that is produced by a type of immune cell called Th17 cells and is involved in the immune response and inflammation. On the other hand, ADAMTS-1, -4, and - 5 are enzymes that are involved in the breakdown of extracellular matrix proteins, including proteoglycans, which are important components of the intestinal wall. This study aimed to evaluate the relationship between interleukin 17 (IL-17 A) cytokine, which plays a role in the pathogenesis of ulcerative colitis, and the inflammation-controlled a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1, -4, and - 5 protein members.
Bowel tissue samples and blood serum from 51 patients with UC and 51 healthy controls were included in this study. mRNA expression levels of the ADAMTS-1, -4, -5, and IL-17 A were analyzed by RT-qPCR, and immunohistochemical analyses were performed to evaluate ADAMTS-1, -4, -5, and IL-17 A proteins in tissue samples. In addition, ELISA analysis determined serum levels of the ADAMTS-1, -4, -5, and IL-17 A.
RT-qPCR results reveal that the expression of ADAMTS-1, -4, -5, and IL-17 A genes in the UC tissue samples were significantly high according to the control tissue samples. Also, ADAMTS-1, -4, -5, and IL-17 A proteins revealed enhanced expression pattern UC groups according to the control. Also, ADAMTS-1, -4, -5, and IL-17 A protein showed cytoplasmic localization patterns in both control and UC groups. The serum levels of ADAMTS-1,-5, and IL-17 A were significantly higher in UC samples than in the control group.
We observed a positive correlation between the ADAMTS-1, -5 and IL17A cytokine expression in UC samples. These results provide a new understanding of controlling crucial ADAMTS family protein members by IL-17 A cytokines with UC.
溃疡性结肠炎（UC）是一种慢性炎症性肠病，由于遗传易感个体免疫反应受损而发展，其发病机制尚未完全阐明。IL-17A 是一种细胞因子，由 Th17 细胞等免疫细胞产生，参与免疫反应和炎症。另一方面，ADAMTS-1、-4 和 -5 是参与细胞外基质蛋白（包括糖胺聚糖等肠道壁的重要成分）分解的酶。本研究旨在评估白细胞介素 17（IL-17A）细胞因子在溃疡性结肠炎发病机制中的作用与炎症控制的解整合素和金属蛋白酶与血小板反应蛋白基序（ADAMTS）-1、-4 和 -5 蛋白成员之间的关系。
本研究纳入了 51 例 UC 患者和 51 例健康对照者的肠组织样本和血清。通过 RT-qPCR 分析 ADAMTS-1、-4、-5 和 IL-17A 的 mRNA 表达水平，并通过免疫组织化学分析评估组织样本中的 ADAMTS-1、-4、-5 和 IL-17A 蛋白。此外，ELISA 分析测定了血清中 ADAMTS-1、-4、-5 和 IL-17A 的水平。
RT-qPCR 结果显示，与对照组织样本相比，UC 组织样本中 ADAMTS-1、-4、-5 和 IL-17A 基因的表达明显升高。此外，与对照相比，UC 组 ADAMTS-1、-4、-5 和 IL-17A 蛋白表达增强。此外，ADAMTS-1、-4、-5 和 IL-17A 蛋白在对照和 UC 组中均显示细胞质定位模式。UC 样本中的 ADAMTS-1、-5 和 IL-17A 血清水平明显高于对照组。
我们观察到 UC 样本中 ADAMTS-1、-5 和 IL17A 细胞因子表达之间存在正相关。这些结果为通过 UC 中 IL-17A 细胞因子控制关键的 ADAMTS 家族蛋白成员提供了新的认识。
