Wattananit Podjana, Yingchutrakul Yodying, Kornkaewrat Kornchai, Mahasawangkul Sittidet, Roytrakul Sittiruk, Pinyopummin Anuchai
Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, Thailand.
Front Vet Sci. 2023 Sep 20;10:1174078. doi: 10.3389/fvets.2023.1174078. eCollection 2023.
Seminal plasma proteins have recently been reported to play a significant role as valuable materials for understanding male reproductive biology, identifying causes of fertility problems, and developing reproductive biomarkers. Proteomic analysis of seminal plasma holds promise in advancing the understanding of male Asian elephant reproductive biology. This study aims to explore seminal plasma proteins of Asian elephants and their probable functions to provide fundamental information about male reproduction in this species. The protein solution from pooled seminal plasma from 10 bulls (a total of 33 ejaculates) was digested into peptides and identified using LC-MS/MS. Out of 986 proteins, 597 were mapped and matched with 58 species in UniProt databases, including . These mapped proteins were mostly involved in binding function, catalytic activity, cellular process, and metabolic process. Only 29 mapped proteins were recognized to be related in reproductive process, mainly associated in spermatogenesis and sperm capacitation. Additionally, several seminal plasma proteins related to fertility or semen quality in other mammals were also found in Asian elephant semen, such as keratin type I, aldose reductase, thrombospondon-1, fibronectin 1, platelet-activating factor acetyl hydrolase, mannosidase, and semenogelin-2. This discovery clearly reveals the beneficial protein profile in seminal plasma of the Asian elephant and serves as a crucial step in investigating infertility and poor semen quality in this valuable species.
近期有报道称，精浆蛋白作为了解雄性生殖生物学、确定生育问题原因以及开发生殖生物标志物的重要材料发挥着重要作用。精浆的蛋白质组学分析有望推动对亚洲象雄性生殖生物学的理解。本研究旨在探索亚洲象的精浆蛋白及其可能的功能，以提供该物种雄性生殖的基础信息。将来自10头雄性亚洲象（共33份精液）的混合精浆的蛋白质溶液消化成肽段，并使用液相色谱 - 串联质谱法（LC - MS/MS）进行鉴定。在986种蛋白质中，有597种在UniProt数据库中与58个物种进行了映射和匹配，其中包括……这些映射的蛋白质大多参与结合功能、催化活性、细胞过程和代谢过程。只有29种映射的蛋白质被认为与生殖过程相关，主要与精子发生和精子获能有关。此外，在亚洲象精液中还发现了几种与其他哺乳动物的生育力或精液质量相关的精浆蛋白，如I型角蛋白、醛糖还原酶、血小板反应蛋白 - 1、纤连蛋白1、血小板活化因子乙酰水解酶、甘露糖苷酶和精液凝胶蛋白 - 2。这一发现清楚地揭示了亚洲象精浆中有益的蛋白质谱，是研究这个珍贵物种不育和精液质量差问题的关键一步。
