Satitmanwiwat S, Promthep K, Buranaamnuay K, Mahasawangkul S, Saikhun K
Reproductive Biology Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology of North Bangkok, Bangkok, Thailand.
Reprod Domest Anim. 2017 Apr;52(2):283-288. doi: 10.1111/rda.12900. Epub 2017 Jan 6.
Peroxidation damage to spermatozoa and seminal plasma has an important role in sperm quality. Thus, the objective of this study was to determine the levels of lipid and protein oxidation in spermatozoa and seminal plasma of Asian elephants (Elephas maximus) with varying percentage of progressive motility. Lipid and protein oxidation was measured by the thiobarbituric acid-reactive species (TBARS) assay and the 2, 4-dinitrophenylhydrazine (DNPH) carbonyl groups assay, respectively. Fresh semen samples were collected from Asian elephants and classified according to the percentage of motile spermatozoa into good (>60%) and poor (≤20%) motility. Results revealed that seminal plasma malondialdehyde (MDA) and seminal plasma protein carbonyls (PCs) were significantly higher in poor motility than in good motility (p < .05). The MDA and PC levels in seminal plasma were negatively correlated with the percentages of progressive motility (p < .05). In addition, the negative correlation between sperm concentration and seminal plasma MDA level was investigated (p < .05). The sperm viability was also negatively correlated with sperm PC level (p < .05). This study indicated that lipid and protein oxidation has deleterious effect on semen quality of Asian elephants.
精子和精浆的过氧化损伤在精子质量中起着重要作用。因此，本研究的目的是确定不同前进运动百分比的亚洲象（印度象）精子和精浆中的脂质和蛋白质氧化水平。脂质和蛋白质氧化分别通过硫代巴比妥酸反应性物质（TBARS）测定法和2,4-二硝基苯肼（DNPH）羰基测定法进行测量。从亚洲象收集新鲜精液样本，并根据活动精子的百分比分为运动性良好（>60%）和运动性差（≤20%）两类。结果显示，运动性差的精浆丙二醛（MDA）和精浆蛋白羰基（PCs）显著高于运动性良好的（p <.05）。精浆中的MDA和PC水平与前进运动百分比呈负相关（p <.05）。此外，还研究了精子浓度与精浆MDA水平之间的负相关（p <.05）。精子活力也与精子PC水平呈负相关（p <.05）。本研究表明，脂质和蛋白质氧化对亚洲象的精液质量有有害影响。
