碱性磷酸酶“片段化”同工酶在暂时性高磷酸酶血症诊断中的应用
"Fragmented" isoenzymes of alkaline phosphatase in the diagnosis of transient hyperphosphatasemia.
作者信息
Schoenau E, Herzog K H, Boehles H J
出版信息
Clin Chem. 1986 Dec;32(12):2211-3.
We describe the appearance of "fragmented" isoenzymes of serum alkaline phosphatase (EC 3.1.3.1) in two cases of transient hyperphosphatasemia. We determined the isoenzymes by liquid chromatography, then characterized them by heat inactivation, inhibition with 5 mmol/L L-phenylalanine solution, and electrophoresis on cellulose acetate membranes. We suspect that a virus-induced decrease in clearance of the enzyme from serum is responsible for a similar increase of bone and liver isoenzyme activities and for the presence of these fragmented isoenzymes in transient hyperphosphatasemia.
我们描述了两例短暂性高磷酸酶血症患者血清碱性磷酸酶（EC 3.1.3.1）“片段化”同工酶的表现。我们通过液相色谱法测定同工酶，然后通过热失活、用5 mmol/L L-苯丙氨酸溶液抑制以及在醋酸纤维素膜上进行电泳对其进行表征。我们怀疑病毒诱导的血清中该酶清除率降低是短暂性高磷酸酶血症中骨和肝同工酶活性类似增加以及这些片段化同工酶存在的原因。
Zotero 插件