Department of Microbiology, University of Alabama at Birmingham , Birmingham, Alabama, USA.
Microbiol Spectr. 2023 Dec 12;11(6):e0219823. doi: 10.1128/spectrum.02198-23. Epub 2023 Oct 6.
Respiratory infections are a leading cause of morbidity and mortality in people with cystic fibrosis (CF). These infections are polymicrobial in nature with overt pathogens and other colonizing microbes present. Microbiome data have indicated that the presence of oral commensal bacteria in the lungs is correlated with improved outcomes. We hypothesize that one oral commensal, inhibits CF pathogens and modulates the host immune response. One major CF pathogen is , a Gram-negative, opportunistic bacterium with intrinsic drug resistance and an arsenal of virulence factors. We have previously shown that inhibits in a nitrite-dependent manner through the production of reactive nitrogen intermediates. In this study, we demonstrate that while this mechanism is evident in a cell culture model of the CF airway, an alternative mechanism by which may improve outcomes for people with CF is through immunomodulation.
呼吸道感染是囊性纤维化 (CF) 患者发病率和死亡率的主要原因。这些感染本质上是多微生物的，存在显性病原体和其他定植微生物。微生物组数据表明，肺部中存在口腔共生细菌与改善结果相关。我们假设一种口腔共生细菌， 通过抑制 CF 病原体和调节宿主免疫反应来发挥作用。一种主要的 CF 病原体是 ，一种革兰氏阴性、机会性细菌，具有内在的耐药性和一系列毒力因子。我们之前已经表明， 通过产生反应性氮中间产物以亚硝酸盐依赖的方式抑制 。在这项研究中，我们证明，虽然这种机制在 CF 气道的细胞培养模型中是明显的，但 通过免疫调节改善 CF 患者预后的另一种机制可能是 。
