Esquivel Jesus F, Joyce Andrea L
US Department of Agriculture, Agricultural Research Service, Insect Control and Cotton Disease Research Unit, 2765 F&B Road, College Station, TX 77845, USA.
University of California Merced, Department of Public Health, 5200 N. Lake Road, Merced, CA 95343, USA.
J Econ Entomol. 2023 Dec 11;116(6):2201-2206. doi: 10.1093/jee/toad177.
The leaffooted bug, Leptoglossus clypealis Heidemann, is a seed-feeding economic pest of crops including almonds and pistachios. The historical distribution of L. clypealis has been considered to be West of the Mississippi in the United States. L. clypealis was recently found in sorghum in the Coastal Bend of Texas, representing a new host record and new collection locality. This study investigated the genetic diversity of L. clypealis museum voucher samples from the Western United States (i.e., Texas, California, and Idaho) collected from 1994 to 2019, including the L. clypealis samples from the Coastal Bend. Eleven new sequences were obtained. Sample sequences were compared with public sequences of L. clypealis from the Western United States. The mitochondrial DNA cytochrome oxidase 1 (mtDNA COI) barcode gene region revealed differences among and within the collection regions. Texas, Idaho, and California all had samples with unique genotypes, and the combined dataset had a haplotype diversity of 1.0. The Texas specimens recently collected in the Coastal Bend did not match genotypes from California or Idaho, and it is unlikely they were recently introduced. Overall, L. clypealis from Texas, Idaho, and California have a high level of genetic diversity, and the 3 regions appear to be within the native range of the species.
叶足蝽象（Leptoglossus clypealis Heidemann）是一种以种子为食的经济害虫，会危害包括杏仁和开心果在内的多种作物。叶足蝽象的历史分布范围被认为是在美国密西西比河以西。最近在得克萨斯州沿海湾地区的高粱中发现了叶足蝽象，这代表了一个新的寄主记录和新的采集地点。本研究调查了1994年至2019年从美国西部（即得克萨斯州、加利福尼亚州和爱达荷州）采集的叶足蝽象博物馆凭证样本的遗传多样性，包括来自沿海湾地区的叶足蝽象样本。获得了11个新序列。将样本序列与来自美国西部的叶足蝽象公共序列进行了比较。线粒体DNA细胞色素氧化酶1（mtDNA COI）条形码基因区域揭示了采集区域之间和区域内的差异。得克萨斯州、爱达荷州和加利福尼亚州都有具有独特基因型的样本，并且合并数据集的单倍型多样性为l.0。最近在沿海湾地区采集的得克萨斯州标本与来自加利福尼亚州或爱达荷州的基因型不匹配，并且它们不太可能是最近引入的。总体而言，来自得克萨斯州、爱达荷州和加利福尼亚州的叶足蝽象具有高度的遗传多样性，并且这三个区域似乎都在该物种的原生范围内。
