Department of Chemistry "Ugo Schiff', University of Florence, 50019, Sesto Fiorentino, FI, Italy.
Department of Chemistry and Applied Biosciences, ETH Zurich, Ramistrasse 101, 8092, Zurich, Switzerland.
Biosens Bioelectron. 2023 Dec 15;242:115713. doi: 10.1016/j.bios.2023.115713. Epub 2023 Sep 30.
Molecular imprinting and related technologies are becoming increasingly appreciated in bioanalysis and diagnostic applications. Among the imprinted polymers, we have already demonstrated that the endogenous neurotransmitters (NTs) dopamine (DA) and norepinephrine (NE) can be efficiently used as natural and sustainable monomers to straightforwardly design and synthesize a new generation of green and "soft" Molecularly Imprinted BioPolymers (MIBPs). Here, we demonstrated for the first time the ability of a further NT, i.e., serotonin (SE), in forming adhesive imprinted nanofilms coupled to label-free optical biosensing. Its imprinting efficiency is compared with those obtained with PDA and PNE. As a model study, tumor necrosis factor-alpha (TNF-α) was selected as a biomolecular target of interest in clinical diagnostics. The biomimetic receptor was coupled to Surface Plasmon Resonance (SPR), and TNF-α detection was performed in label-free and real-time manner both in buffer and biological matrices, i.e. synovial fluid and human serum. The results indicate that, under the same imprinting and binding conditions, the analytical performances of PSE are impressively superior to those of PDA and PNE. The PSE-based MIBP was able to detect TNF-α in human matrices with a good sensitivity, selectivity, and repeatability.
分子印迹技术和相关技术在生物分析和诊断应用中越来越受到重视。在印迹聚合物中，我们已经证明内源性神经递质（NTs）多巴胺（DA）和去甲肾上腺素（NE）可以有效地用作天然和可持续的单体，直接设计和合成新一代绿色和“软”的分子印迹生物聚合物（MIBP）。在这里，我们首次证明了另一种 NT，即 5-羟色胺（SE），在形成与无标记光学生物传感偶联的粘性印迹纳米薄膜方面的能力。其印迹效率与 PDA 和 PNE 获得的印迹效率进行了比较。作为模型研究，肿瘤坏死因子-α（TNF-α）被选为临床诊断中感兴趣的生物分子靶标。仿生受体与表面等离子体共振（SPR）偶联，并以无标记和实时方式在缓冲液和生物基质（即滑液和人血清）中进行 TNF-α检测。结果表明，在相同的印迹和结合条件下，PSE 的分析性能明显优于 PDA 和 PNE。基于 PSE 的 MIBP 能够以良好的灵敏度、选择性和重复性检测人基质中的 TNF-α。
