Wu Si-Wei, Cheng Cai-Qin, Huang Yi-Tong, Tan Jin-Zhou, Li Song-Liang, Yang Jia-Xin, Huang Xue-Ling, Huang Dan, Zou Li-Gong, Yang Wei-Dong, Li Hong-Ye, Li Da-Wei
Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
The First People's Hospital of Qinzhou, The Tenth Affiliated Hospital of Guangxi Medical University, China.
J Hazard Mater. 2024 Jan 5;461:132624. doi: 10.1016/j.jhazmat.2023.132624. Epub 2023 Sep 24.
Extensive application of organophosphorus pesticides such as phenthoate results in its abundance in ecosystems, particularly in waterbodies, thereby providing the impetus to assess its role in aquatic organisms. However, the impact of phenthoate on marine algal physiological and proteomic response is yet to be explored despite its biological significance. In this study, we thus ought to investigate the impact of phenthoate in the marine dinoflagellate Prorocentrum lima, which is known for synthesizing okadaic acid (OA), the toxin responsible for diarrhetic shellfish poisoning (DSP). Our results showed that P. lima effectively absorbed phenthoate in seawater, with a reduction efficiency of 90.31% after 48 h. Surprisingly, the provision of phenthoate (100 and 1000 µg/L) substantially reduced the OA content of P. lima by 35.08% and 60.28% after 48 h, respectively. Meanwhile, phenthoate treatment significantly reduced the oxidative stress in P. lima. Proteomic analysis revealed that the expression level of seven crucial proteins involved in endocytosis was upregulated, suggesting that P. lima could absorb phenthoate via the endocytic signaling pathway. Importantly, phenthoate treatment resulted in the downregulation of proteins such as polyketide synthase (PKS)- 2, Cytochrome P450 (CYP450)- 1, and CYP450-2, involved in OA synthesis, thereby decreasing the OA biosynthesis by P. lima. Our results demonstrated the potential role of P. lima in the removal of phenthoate in water and exemplified the crucial proteins and their possible molecular mechanisms underpinning the phenthoate remediation by P. lima and also the regulatory role of phenthoate in restricting the OA metabolism. Collectively, these findings uncovered the synergistic mechanisms of phenthoate and P. lima in remediating phenthoate and reducing the toxic impact of P. lima.
诸如稻丰散等有机磷农药的广泛应用导致其在生态系统中大量存在，尤其是在水体中，从而促使人们评估其在水生生物中的作用。然而，尽管稻丰散具有生物学意义，但其对海洋藻类生理和蛋白质组反应的影响尚未得到探索。因此，在本研究中，我们旨在调查稻丰散对海洋甲藻利马原甲藻的影响，该甲藻以合成冈田酸（OA）而闻名，冈田酸是导致腹泻性贝类中毒（DSP）的毒素。我们的结果表明，利马原甲藻能有效吸收海水中的稻丰散，48小时后去除效率达90.31%。令人惊讶的是，添加稻丰散（100和1000µg/L）48小时后，利马原甲藻的OA含量分别大幅降低了35.08%和60.28%。同时，稻丰散处理显著降低了利马原甲藻的氧化应激。蛋白质组分析显示，参与内吞作用的七种关键蛋白质的表达水平上调，这表明利马原甲藻可以通过内吞信号通路吸收稻丰散。重要的是，稻丰散处理导致参与OA合成的聚酮合酶（PKS）-2、细胞色素P450（CYP450）-1和CYP450-2等蛋白质的表达下调，从而减少了利马原甲藻的OA生物合成。我们的结果证明了利马原甲藻在去除水中稻丰散方面的潜在作用，并举例说明了支持利马原甲藻修复稻丰散的关键蛋白质及其可能的分子机制，以及稻丰散在限制OA代谢方面的调节作用。总的来说，这些发现揭示了稻丰散和利马原甲藻在修复稻丰散和降低利马原甲藻毒性影响方面的协同机制。
