基于超高效液相色谱-四极杆飞行时间串联质谱、网络药理学和“双外标”定量分析质谱法的复方车前草片质量评价
[Quality evaluation of Compound Cheqian Tablets based on UPLC-Q-TOF-MS/MS, network pharmacology and "double external standards" QAMS].
作者信息
Wang Kang, Liu Pei, Wang Si-Fan, Zhang Jie-Yu, Hu Zhi-Zhi, Mei Yu-Qi, Yang Ying-Bo, Wang Zheng-Tao, Yang Li
机构信息
the MOE Key Laboratory for Standardization of Chinese Medicines and SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China Shanghai R&D Centre for Standardization of Chinese Medicines Shanghai 201203, China Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
the MOE Key Laboratory for Standardization of Chinese Medicines and SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China School of Pharmacy,Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2023 Sep;48(17):4675-4685. doi: 10.19540/j.cnki.cjcmm.20230605.302.
The Compound Cheqian Tablets are derived from Cheqian Power in Comprehensive Recording of Divine Assistance, and they are made by modern technology with the combination of Plantago asiatica and Coptis chinensis. To investigate the material basis of Compound Cheqian Tablets in the treatment of diabetic nephropathy, in this study, the chemical components of Compound Cheqian Tablets were characterized and analyzed by UPLC-Q-TOF-MS/MS, and a total of 48 chemical components were identified. The identified chemical compounds were analyzed by network pharmacology. By validating with previous literature, six bioactive compounds including acteoside, isoacteoside, coptisine, magnoflorine, palmatine, and berberine were confirmed as the index components for qua-lity evaluation. Furthermore, the content of the six components in the Compound Cheqian Tablets was determined by the "double external standards" quantitative analysis of multi-components by single marker(QAMS), and the relative correction factor of isoacteoside was calculated as 1.118 by using acteoside as the control; the relative correction factors of magnoflorine, palmatine, and berberine were calculated as 0.729, 1.065, and 1.126, respectively, by using coptisine as the control, indicating that the established method had excellent stability under different conditions. The results obtained by the "double external standards" QAMS approximated those obtained by the external standard method. This study qualitatively characterized the chemical components in the Compound Cheqian Tablets by applying UPLC-Q-TOF-MS/MS and screened the pharmacodynamic substance basis for the treatment of diabetic nephropathy via network pharmacology, and primary pharmacodynamic substance groups were quantitatively analyzed by the "double external stan-dards" QAMS method, which provided a scientific basis for clarifying the pharmacodynamic substance basis and quality control of Compound Cheqian Tablets.
复方车前草片源自《神效万全方》中的车前草散，采用现代技术将车前草与黄连配伍制成。为探究复方车前草片治疗糖尿病肾病的物质基础，本研究采用超高效液相色谱-四极杆飞行时间串联质谱（UPLC-Q-TOF-MS/MS）对复方车前草片的化学成分进行表征和分析，共鉴定出48种化学成分。对鉴定出的化合物进行网络药理学分析。通过与既往文献验证，确定了毛蕊花糖苷、异毛蕊花糖苷、黄连碱、吴茱萸碱、巴马汀和小檗碱6种生物活性化合物作为质量评价的指标成分。此外，采用“双外标”多成分单指标定量分析方法（QAMS）测定复方车前草片中6种成分的含量，以毛蕊花糖苷为对照，计算异毛蕊花糖苷的相对校正因子为1.118；以黄连碱为对照，计算吴茱萸碱、巴马汀和小檗碱的相对校正因子分别为0.729、1.065和1.126，表明所建立的方法在不同条件下具有良好的稳定性。“双外标”QAMS法所得结果与外标法所得结果相近。本研究应用UPLC-Q-TOF-MS/MS对复方车前草片中的化学成分进行定性表征，通过网络药理学筛选出治疗糖尿病肾病的药效物质基础，并采用“双外标”QAMS法对主要药效物质组进行定量分析，为阐明复方车前草片的药效物质基础和质量控制提供了科学依据。
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