Wang Liyuan, Mao Hongmei, Shen Shi, Chen Xi, Wang Tonglei, Zhao Jinpeng, Han Chao, Huo Junsheng
National Institute for Nutrition and Health Chinese Center for Disease Control and Prevention, NHC Key Laboratory of Trace Element Nutrition, Beijing 100050, China.
Wei Sheng Yan Jiu. 2023 Sep;52(5):749-755. doi: 10.19813/j.cnki.weishengyanjiu.2023.05.010.
To investigate the repairing effect of Lactobacillus acidophilus LA11-Only on ceftriaxone induced intestinal micronbiota imbalance in mice and its alleviating effect on lipid metabolism.
A total of 30 SPF BALB/c male mice were randomly divided into control group(10) and model group(20). The model group was given ceftriaxone sodium solution by gavage for a week to create a mouse model of antibiotic flora disorder. The natural recovery group(10) was given 10% skimmed milk solution by gavage, and the LA11-Onlly group was given Lactobacillus acidophilus skimmed milk solution(1.0×10~9CFU/mL). Two weeks later, the feces were collected aseptically.16S rRNA sequencing technology and ultra-high-performance liquid chromatography combined with mass spectrometry(UPLC-MS) were used to analyze the repair effect of LA11-Onlly on antibiotic induced bacterial flora disorder and lipid metabolism in mice.
After ceftriaxone treatment, the diversity of intestinal microbiota in mice decreased, and there was a significant difference in the composition of flora compared with the control group; After treatment with LA11-Onlly, the Alpha diversity increased, the abundance of beneficial bacteria such as Lactobacillus and Butyricicoccus increased, and the composition of flora was closer to the control group; At the same time, the concentration of short chain fatty acids(SCFAs) increased in varying degrees. Compared with the natural recovery group, the levels of butyric acid and valeric acid in LA11-Onlly group increased significantly, close to the same level as the control group.
Lactobacillus acidophilus LA11-Onlly probiotics can inhibit the growth of harmful bacteria by regulating the diversity and community composition of intestinal microbiota, promote the increase of the concentration of SCFAs, and alleviate the damage of antibiotics to the body.
研究嗜酸乳杆菌LA11-Only对头孢曲松诱导的小鼠肠道微生物群失衡的修复作用及其对脂质代谢的缓解作用。
将30只SPF级BALB/c雄性小鼠随机分为对照组（10只）和模型组（20只）。模型组连续一周灌胃头孢曲松钠溶液以建立抗生素菌群失调小鼠模型。自然恢复组（10只）灌胃10%脱脂乳溶液，LA11-Only组灌胃嗜酸乳杆菌脱脂乳溶液（1.0×10⁹CFU/mL）。两周后，无菌收集粪便。采用16S rRNA测序技术和超高效液相色谱-质谱联用（UPLC-MS）分析LA11-Only对小鼠抗生素诱导的菌群失调和脂质代谢的修复作用。
头孢曲松处理后，小鼠肠道微生物群多样性降低，菌群组成与对照组相比有显著差异；LA11-Only处理后，Alpha多样性增加，乳酸杆菌和丁酸球菌等有益菌丰度增加，菌群组成更接近对照组；同时，短链脂肪酸（SCFAs）浓度不同程度升高。与自然恢复组相比，LA11-Only组丁酸和戊酸水平显著升高，接近对照组水平。
嗜酸乳杆菌LA11-Only益生菌可通过调节肠道微生物群的多样性和群落组成抑制有害菌生长，促进SCFAs浓度升高，减轻抗生素对机体的损害。
