Group of Chemistry and Biochemistry of Pigments, Instituto de la Grasa (CSIC), Building 46, 41013 Sevilla, Spain.
Food Lipid Biomarkers and Health Group, Institute of Food Science Research (CSIC-UAM), 28049 Madrid, Spain.
Food Res Int. 2023 Nov;173(Pt 1):113330. doi: 10.1016/j.foodres.2023.113330. Epub 2023 Aug 1.
The milk fat globule membrane (MFGM) provides infants and adults with several health benefits. These are not derived solely from its unique composition, but also from arrangement of lipids in the MFGM that, in the case of newborns, could reach the intestine partially intact. Fluorochromes associated with lipid derivatives were used to prove a fusion process between the MFGM and the cellular membrane of differentiated Caco-2 cells. To explore the mechanism of this interaction, incubations of MFGM with Caco-2 cells were carried out in the presence of fusogenic agents or compounds that block other MFGM interaction pathways with cells. Confocal fluorescence microscopy provided visual evidence of the fusion process. Lastly, determination on the lipid profile of cells after their interaction with MFGM indicated a metabolic rearrangement of lipids leading to accumulation of triacylglycerols.
牛奶脂肪球膜 (MFGM) 为婴儿和成人提供了多种健康益处。这些益处不仅源自其独特的组成，还源自 MFGM 中脂质的排列方式，在新生儿的情况下，这些脂质排列方式可以使 MFGM 部分完整地到达肠道。与脂质衍生物相关的荧光染料被用于证明 MFGM 与分化的 Caco-2 细胞的细胞膜之间存在融合过程。为了探索这种相互作用的机制，在存在融合试剂或阻断 MFGM 与细胞其他相互作用途径的化合物的情况下，进行了 MFGM 与 Caco-2 细胞的孵育。共聚焦荧光显微镜为融合过程提供了直观的证据。最后，对与 MFGM 相互作用后的细胞的脂质谱进行测定表明，脂质发生了代谢重排，导致三酰基甘油的积累。
