Group of Chemistry and Biochemistry of Pigments, Instituto de la Grasa (CSIC), Building 46, 41013 Sevilla, Spain.
Food Lipid Biomarkers and Health Group, Institute of Food Science Research (CSIC-UAM), 28049 Madrid, Spain.
Nutrients. 2024 Aug 22;16(16):2798. doi: 10.3390/nu16162798.
This study aimed to evaluate the digestive efficiency of food matrices supplemented with milk fat globule membrane isolated from buttermilk (BM-MFGM), using the INFOGEST in vitro digestion protocol hyphenated with the assessment of the digested material on the lipid profile of the Caco-2 cell culture model. First, we examined lipid profiles in food matrices supplemented with BM-MFGM and their subsequent digestion. The results showed distinct lipid profiles in different food matrices and micellar fractions. The presence of BM-MFGM lipids changed the cellular lipid profiles in Caco-2 cell cultures, with diverging contents in cholesteryl esters, triacylglycerides, and neutral lipids depending on the micellar food matrix factor. Hierarchical clustering analysis revealed patterns in cellular lipid responses to micellar stimuli, while volcano plots highlighted significant changes in cellular lipid profiles post-treatment. Thus, this study underscores the importance of in vitro digestion protocols in guiding food matrix selection for bioactive ingredient supplementation, elucidating intestinal epithelium responses to digested food stimuli.
本研究旨在评估添加了从白脱牛奶中分离的乳脂肪球膜（BM-MFGM）的食物基质的消化效率，使用 INFOGEST 体外消化方案，结合对 Caco-2 细胞培养模型中消化材料的脂质谱评估。首先，我们检查了添加 BM-MFGM 的食物基质及其随后的消化的脂质谱。结果表明，不同食物基质和胶束级分中的脂质谱存在明显差异。BM-MFGM 脂质的存在改变了 Caco-2 细胞培养物中的细胞脂质谱，根据胶束食物基质因素，胆固醇酯、三酰基甘油和中性脂质的含量存在差异。层次聚类分析显示了细胞脂质对胶束刺激的反应模式，而火山图则突出了处理后细胞脂质谱的显著变化。因此，本研究强调了体外消化方案在指导用于生物活性成分补充的食物基质选择方面的重要性，阐明了肠道上皮细胞对消化食物刺激的反应。
