代谢组学的动态分析揭示了结肠肽与血清食欲相关激素之间的潜在关联。

Dynamic analysis of metabolomics reveals the potential associations between colonic peptides and serum appetite-related hormones.

机构信息

Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Food Res Int. 2023 Nov;173(Pt 2):113376. doi: 10.1016/j.foodres.2023.113376. Epub 2023 Aug 18.

DOI:10.1016/j.foodres.2023.113376

PMID:37803714

Abstract

Gut signals, including hormones and metabolites are crucial zeitgebers that regulate the circadian rhythm of host metabolism, but the potential links have been explored more in rodents. Herein, we performed an hour-scale metabolomics analysis of serum and colonic digesta to characterize the circadian rhythmic metabolic patterns using a pig model under ad libitum feeding conditions. Importantly, our findings identified potential associations between colonic and body metabolism, revealing the potential relationships between colonic peptides and host appetite regulation. Concretely, amino acids accounted for the highest proportion in rhythmic serum metabolites, whereas lipids accounted for the highest proportion in rhythmic colonic metabolites. The diurnal difference analysis revealed that the levels of most amino acids and peptides were higher in the light phase, while the levels of most lipids were higher in the dark phase. And more correlations were be checked between serum amino acids, lipids, peptides and colonic metabolites in the light and more correlations were be checked between serum carbohydrates, cofactors and vitamins, energy, nucleotides, xenobiotics and colonic metabolites in the dark. Interestingly, peptides oscillated to a similar extent in serum and colonic digesta. Of note, colonic peptides composed of valine, proline and leucine were checked in positive associations to glucagon-like peptide-1 (GLP-1) in serum. And these peptides were positive with the genera Butyricicoccus, Streptococcus, Clostridioides, Bariatricus and Coriobacteriia_norank, and negative with Prevotella, and showed the potential relationships with colonic microbial biosynthesis of amino acids. Collectively, we mapped the rhythmic profiling on pig serum and colonic metabolites and revealed the relationships between host and gut metabolism. However, the underlying regulatory mechanisms remains to be further investigated.

摘要

肠道信号，包括激素和代谢物，是调节宿主代谢昼夜节律的重要生物钟，但这些潜在的联系在啮齿动物中得到了更多的探索。在此，我们使用自由采食条件下的猪模型，对血清和结肠消化物进行了小时尺度的代谢组学分析，以表征昼夜节律代谢模式。重要的是，我们的研究结果确定了结肠和身体代谢之间的潜在关联，揭示了结肠肽与宿主食欲调节之间的潜在关系。具体而言，在节律性血清代谢物中，氨基酸占比最高，而在节律性结肠代谢物中，脂质占比最高。昼夜差异分析显示，大多数氨基酸和肽的水平在光照期较高，而大多数脂质的水平在暗期较高。并且在光照期，血清中的氨基酸、脂质、肽和结肠代谢物之间有更多的相关性，而在暗期，血清中的碳水化合物、辅因子和维生素、能量、核苷酸、外源性化合物和结肠代谢物之间有更多的相关性。有趣的是，肽在血清和结肠消化物中的波动程度相似。值得注意的是，由缬氨酸、脯氨酸和亮氨酸组成的结肠肽与血清中的胰高血糖素样肽-1（GLP-1）呈正相关。这些肽与丁酸球菌属、链球菌属、梭菌属、Bariatricus 属和 Coriobacteriia_norank 呈正相关，与普雷沃氏菌属呈负相关，显示出与结肠微生物氨基酸生物合成的潜在关系。总的来说，我们绘制了猪血清和结肠代谢物的节律图谱，揭示了宿主和肠道代谢之间的关系。然而，潜在的调节机制仍有待进一步研究。