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[与弓形虫抗原的乳胶反应]

[Latex reaction with toxoplasma antigen].

作者信息

Bozdĕch V

出版信息

Angew Parasitol. 1979 Feb;20(1):1-8.

PMID:378039
Abstract

The author describes the latex fixation test (LFT) with Toxoplasma antigen. The main parts of this technique are as follows: the sensibilization of latex particles and incubation of the sensitized particles with sera examined in an icebox (overnight) followed by spinning at 5000 G 10 minutes.--The sera giving negative results with LRT reacted also in 78,3% with complement-fixing test (CFT) in 79.3% and with indirect fluorescent test (IFAT) in 61.8% and with microprecipitating test (MPT) in 100% negatively.--The sera reacting strongly positively with LFT reacted also with CFT in high titers (titer 160 and higher) in 60.9% and in 55% with IFAT (titers 128 and higher).

摘要

作者描述了用弓形虫抗原进行的乳胶凝集试验(LFT)。该技术的主要步骤如下:使乳胶颗粒致敏,将致敏颗粒与待检测血清在冰箱中孵育(过夜),然后以5000g离心10分钟。——LRT检测结果为阴性的血清,补体结合试验(CFT)的阳性反应率为78.3%,间接荧光试验(IFAT)的阳性反应率为79.3%,微沉淀试验(MPT)的阳性反应率为61.8%,而MPT的阴性反应率为100%。——LFT检测结果为强阳性的血清,CFT高滴度(滴度160及以上)的阳性反应率为60.9%,IFAT(滴度128及以上)的阳性反应率为55%。

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