Pereira Vanessa Ingrid Cardoso, de Brito Junior Lacy Cardoso, Falcão Luiz Fabio Magno, da Costa Vasconcelos Pedro Fernando, Quaresma Juarez Antônio Simões, Berg Ana Virgínia Van Dem, Paixão Ana Paula Silveira, Ferreira Roberta Isabella Senna, Diks Izabela Bezerra Costa
Clinical Pathology Laboratory Dr Paulo C Azevedo, Belém, Pará, Brasil.
Institute of Biological Sciences at UFPA. Laboratory of General Pathology - Immunopathology and Cytology at FederalUniversity of Pará. Belém, Pará, Brazil.
Int Immunopharmacol. 2023 Nov;124(Pt B):110994. doi: 10.1016/j.intimp.2023.110994. Epub 2023 Oct 5.
The present study sought to characterize the pattern of monocyte subpopulations in patients during the course of the infections caused by SARS-CoV-2 virus or who presented long COVID-19 syndrome compared to monocytes from patients with zika virus (Zika) or chikungunya virus (CHIKV).
Study with 89 peripheral blood samples from patients, who underwent hemogram and serology (IgG and IgM) for detection of Zika (Control Group 1, n = 18) or CHIKV (Control Group 2, n = 9), and from patients who underwent hemogram and reverse transcription polymerase chain reaction for detection of SARS-CoV-2 at the acute phase of the disease (Group 3, n = 19); and of patients who presented long COVID-19 syndrome (Group 4, n = 43). The monocyte and subpopulations counts were performed by flow cytometry.
No significant difference was observed in the total number of monocytes between the groups. The classical (CD14CD16) and intermediate (CD14CD16) monocytes counts were increased in patients with acute infection or with long COVID-19 syndrome. The monocytes subpopulations counts were lower in patients with infection Zika or CHIKV.
Increase in the monocyte subpopulations in patients with acute infection or with long COVID-19 syndrome may be an important finding of differentiated from the infection Zika or CHIKV.
本研究旨在描述感染严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒的患者或患有新冠后综合征的患者体内单核细胞亚群的模式，并与感染寨卡病毒（Zika）或基孔肯雅病毒（CHIKV）的患者的单核细胞进行比较。
对89例患者的外周血样本进行研究，这些患者接受了血常规和血清学检查（IgG和IgM）以检测寨卡病毒（对照组1，n = 18）或基孔肯雅病毒（对照组2，n = 9），以及在疾病急性期接受血常规和逆转录聚合酶链反应以检测SARS-CoV-2的患者（第3组，n = 19）；还有患有新冠后综合征的患者（第4组，n = 43）。通过流式细胞术进行单核细胞及其亚群计数。
各组之间单核细胞总数未观察到显著差异。在急性感染患者或患有新冠后综合征的患者中，经典型（CD14⁺CD16⁻）和中间型（CD14⁺CD16⁺）单核细胞计数增加。感染寨卡病毒或基孔肯雅病毒的患者单核细胞亚群计数较低。
急性感染患者或患有新冠后综合征的患者单核细胞亚群增加可能是与感染寨卡病毒或基孔肯雅病毒相区别的一个重要发现。
