Department of Chemistry and Liver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
Institutes of Biomedical Sciences and NHC Key Laboratory of Glycoconjugates Research, Fudan University, Shanghai, 200032, China.
Talanta. 2024 Jan 15;267:125262. doi: 10.1016/j.talanta.2023.125262. Epub 2023 Oct 4.
Herein, we introduced ortho-phthalaldehyde (OPA) for blocking free amino groups and established a simple and robust method for comprehensive profiling of protein terminome based on strong cation exchange chromatography (SCX) fractionation. With the highly efficient and chemoseletive amine-group blocking, we identified 2271 canonical human protein N-termini, 1650 canonical human protein C-termini, as well as 645 protein neo-N-termini from HeLa cells.
在这里，我们引入邻苯二甲醛（OPA）来封闭游离的氨基，并建立了一种基于强阳离子交换色谱（SCX）分级的简单而强大的蛋白质末端组全面分析方法。通过高效和选择性的胺基封闭，我们鉴定了 2271 个人类蛋白质的 C 端，1650 个人类蛋白质的 N 端，以及 HeLa 细胞中的 645 个蛋白质新 N 端。
