Xiao Fan, Sun Mengze, Zhang Liyun, Lei Xiaoguang
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
Institute for Cancer Research, Shenzhen Bay Laboratory, Shenzhen, 518107, China.
J Org Chem. 2024 Oct 18;89(20):14619-14624. doi: 10.1021/acs.joc.3c01397. Epub 2023 Aug 22.
-Phthalaldehyde (OPA) with high reactivity to the amine group has been widely used to modify proteins. We discovered new modifications of OPA and 2-acylbenzaldehyde and proposed the reaction mechanism. Using isotope labeling mass spectrometry-based experiment, we identified new cross-linking properties of OPA and 2-acylbenzaldehyde. This reactivity revealed that OPA has the potential to probe proximal amino acids in biological systems.
对氨基具有高反应活性的邻苯二甲醛（OPA）已被广泛用于修饰蛋白质。我们发现了OPA和2-酰基苯甲醛的新修饰，并提出了反应机制。通过基于同位素标记质谱的实验，我们确定了OPA和2-酰基苯甲醛的新交联特性。这种反应活性表明OPA有潜力探测生物系统中相邻的氨基酸。
