College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 5):127273. doi: 10.1016/j.ijbiomac.2023.127273. Epub 2023 Oct 5.
Food poisoning caused by Staphylococcus aureus (S. aureus) contaminated meat has received a lot of attention. Although juglone has anti-S. aureus properties, its limited water solubility prevents it from being used in food manufacturing. Juglone @ chitosan nanoemulsion (NJ) was produced for the first time in order to increase its solubility. At the same time, it was applied to the pork model. According to the findings, NJ's particle size was 119.30 nm, its polymer dispersity index (PDI) value was 0.290, and its zeta potential was -57.3 mV. And it's stable over a 7-day storage period. The cell shape and membrane integrity of S. aureus were significantly damaged by NJ. At the same time, NJ showed extreme vigor for biofilm removal. The inclusion of NJ coating significantly reduced S. aureus, total volatile base nitrogen (TVB-N), total viable count (TVC), thiobarbituric acid reactants (TBARS), and pH in the sample when using the pork feeding model. NJ, meantime, halted the sensory evaluation's fall in meat score. Additionally, NJ demonstrated good biocompatibility in mouse acute toxicity tests. The aforementioned findings demonstrate that NJ is anticipated to become an anti-S. aureus and a novel method for coating pork preservation.
金黄色葡萄球菌（S. aureus）污染肉引起的食物中毒受到了广泛关注。虽然胡桃醌具有抗金黄色葡萄球菌的特性，但由于其水溶性有限，无法应用于食品制造。为提高其水溶性，首次制备了胡桃醌@壳聚糖纳米乳液（NJ）。同时，将其应用于猪肉模型中。结果表明，NJ 的粒径为 119.30nm，其聚合物分散指数（PDI）值为 0.290，zeta 电位为-57.3mV。并且在 7 天的储存期内保持稳定。NJ 显著破坏了金黄色葡萄球菌的细胞形态和膜完整性。同时，NJ 对生物膜的去除表现出极强的活力。使用猪肉喂养模型时，NJ 涂层的加入显著降低了样品中的金黄色葡萄球菌、总挥发性碱基氮（TVB-N）、总活菌数（TVC）、硫代巴比妥酸反应物（TBARS）和 pH 值。同时，NJ 阻止了肉品评分的感官评价下降。此外，NJ 在小鼠急性毒性试验中表现出良好的生物相容性。上述结果表明，NJ 有望成为一种抗金黄色葡萄球菌的新型猪肉保鲜方法。
