Dziedzic D, White H J
Environ Res. 1986 Dec;41(2):598-609. doi: 10.1016/s0013-9351(86)80154-2.
Ozone is an oxidant gas which primarily injures the centroacinar portion of the lung. While the classical lesion of oxidant-mediated lung damage is relatively well described, the effect of this form of injury on the lymphocytic arm of the pulmonary defense system is less clear. In the present experiments we exposed CD-1 female mice to ozone at a level of 0.7 ppm for 20 hr per day for 1-28 days and observed the lymphocyte response in the pulmonary lymph nodes and the thymus. In the mediastinal lymph nodes we observed a marked hyperplastic response that was prominent in the paracortex and was characterized by the presence of blastic forms. In contrast, the thymus underwent an atrophic response characterized by cellular loss in the cortical region. Prior surgical adrenalectomy of ozone-exposed animals eliminated part, but not all of the thymic atrophy response, indicating that adrenal-mediated stress alone did not account for all of the observed effect. Thymectomy of animals prior to ozone exposure produced a 40% reduction in the mediastinal lymph node response, suggesting that a part of the node hyperplasia is thymus dependent. The results of these experiments indicate that lymphoid organs are altered following oxidant-mediated lung damage in the mouse. The changes are observed in the absence of exogenous antigenic stimulation and suggest that lymphoid cells are an integral aspect of the host response to high-level ozone inhalation.
臭氧是一种氧化性气体，主要损伤肺的中心腺泡部分。虽然氧化介导的肺损伤的经典病变已有相对充分的描述，但这种损伤形式对肺部防御系统淋巴细胞部分的影响尚不清楚。在本实验中，我们将CD-1雌性小鼠每天暴露于0.7 ppm的臭氧环境中，持续20小时，共暴露1 - 28天，并观察肺淋巴结和胸腺中的淋巴细胞反应。在纵隔淋巴结中，我们观察到明显的增生反应，该反应在副皮质区尤为突出，其特征是存在母细胞形式。相比之下，胸腺经历了萎缩反应，其特征是皮质区域细胞丢失。对暴露于臭氧的动物预先进行手术切除肾上腺，消除了部分但不是全部的胸腺萎缩反应，这表明仅肾上腺介导的应激并不能解释所有观察到的效应。在臭氧暴露前对动物进行胸腺切除，导致纵隔淋巴结反应降低40%，这表明部分淋巴结增生依赖于胸腺。这些实验结果表明，在小鼠中，氧化介导的肺损伤后淋巴器官会发生改变。这些变化是在没有外源性抗原刺激的情况下观察到的，这表明淋巴细胞是宿主对高浓度臭氧吸入反应的一个组成部分。
