Ichihara Y, Sogawa K, Morohashi K, Fujii-Kuriyama Y, Takahashi K
Eur J Biochem. 1986 Nov 17;161(1):7-12. doi: 10.1111/j.1432-1033.1986.tb10117.x.
A nearly full-length rat pepsinogen cDNA was isolated from a rat gastric mucosa cDNA library and its nucleotide sequence was determined. The cDNA comprises 1370 base pairs (bp), including the 5'-non-coding region (60 bp), the coding nucleotide sequence (1176 bp) and the 3'-non-coding region (131 bp). The predicted amino acid sequence of rat prepepsinogen (392 residues) contains a 16-residue signal sequence followed by the pepsionogen moiety of 376 residues. Rat pepsinogen has an amino acid composition characteristic of C-type pepsinogens and is much more homologous in amino acid sequence with C-type pepsinogens than A-type pepsinogens. These results indicate that the major form of rat pepsinogen can be classified as a C type pepsinogen.
从大鼠胃黏膜cDNA文库中分离出一个几乎全长的大鼠胃蛋白酶原cDNA，并测定了其核苷酸序列。该cDNA由1370个碱基对（bp）组成，包括5'-非编码区（60 bp）、编码核苷酸序列（1176 bp）和3'-非编码区（131 bp）。大鼠前胃蛋白酶原的预测氨基酸序列（392个残基）包含一个16个残基的信号序列，其后是376个残基的胃蛋白酶原部分。大鼠胃蛋白酶原具有C型胃蛋白酶原的氨基酸组成特征，并且在氨基酸序列上与C型胃蛋白酶原的同源性远高于A型胃蛋白酶原。这些结果表明，大鼠胃蛋白酶原的主要形式可归类为C型胃蛋白酶原。
