Cui Xiaoqing, Liu Yangyang, Zhang Jingtian, Hu Panpan, Zheng Zheng, Deng Xiaoling, Xu Meirong
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2023 Sep 22;14:1236731. doi: 10.3389/fmicb.2023.1236731. eCollection 2023.
" Liberibacter asiaticus" (CLas) is a notorious agent that causes Citrus Huanglongbing (HLB), which is transmitted by (). We recently found that the acquisition and transmission of CLas by was facilitated by (CTV), a widely distributed virus in the field. In this study, we further studied whether different CTV strains manipulate the host preference of , and whether endosymbionts variation is related to CTV strains in . The results showed that the non-viruliferous preferred to select the shoots infected with CTV, without strain differences was observed in the selection. However, the viruliferous prefered to select the mixed strain that is similar to the field's. Furthermore, effectively acquired the CTV within 2-12 h depending on the strains of the virus. The persistence period of CTV in was longer than 24 days, without reduction of the CTV titers being observed. These results provide a foundation for understanding the transmission mode of on CTV. During the process of CTV acquisition and persistence, the titers of main endosymbionts in showed similar variation trend, but their relative titers were different at different time points. The titers of the " Profftella armatura" and CTV tended to be positively correlated, and the titers of and " Carsonella ruddii" were mostly negatively related with titers of CT31. These results showed the relationship among , endosymbionts, and CTV and provided useful information for further research on the interactions between and CLas, which may benefit the development of approaches for the prevention of CLas transmission and control of citrus HLB.
亚洲韧皮杆菌（CLas）是一种臭名昭著的病原体，可引发柑橘黄龙病（HLB），该病通过（）传播。我们最近发现，一种田间广泛分布的病毒——柑橘衰退病毒（CTV）促进了CLas由（）获取和传播。在本研究中，我们进一步研究了不同的CTV株系是否会操控（）的宿主偏好，以及内共生菌的变异是否与（）中的CTV株系有关。结果表明，无毒的（）更倾向于选择感染CTV的嫩梢，在选择上未观察到株系差异。然而，带毒的（）更倾向于选择与田间相似的混合株系。此外，（）能在2 - 12小时内根据病毒株系有效地获取CTV。CTV在（）中的持续时间超过24天，未观察到CTV滴度降低。这些结果为理解（）在CTV上的传播模式奠定了基础。在CTV获取和持续存在的过程中，（）中主要内共生菌的滴度呈现出相似的变化趋势，但在不同时间点其相对滴度有所不同。“盔甲普罗夫特菌”的滴度与CTV倾向于呈正相关，而（）和“鲁迪卡氏菌”的滴度大多与CT31的滴度呈负相关。这些结果揭示了（）、内共生菌和CTV之间的关系，并为进一步研究（）与CLas之间的相互作用提供了有用信息，这可能有助于开发预防CLas传播和控制柑橘黄龙病的方法。
