分化中的L6大鼠成肌细胞中肌肉特异性mRNA的细胞骨架关联

Cytoskeletal association of muscle-specific mRNAs in differentiating L6 rat myoblasts.

作者信息

Bagchi T, Larson D E, Sells B H

出版信息

Exp Cell Res. 1987 Jan;168(1):160-72. doi: 10.1016/0014-4827(87)90425-3.

Abstract

The importance of the cytoskeleton in protein synthesis was studied in differentiating L6 rat myoblasts. Soluble and cytoskeletal fractions obtained after gentle, non-ionic detergent lysis of myoblasts and myotubes were analysed for the presence of ribosomes and mRNPs. Polysomal mRNPs were predominantly associated with the cytoskeletal framework and free mRNPs were present in both soluble and cytoskeletal fractions. An examination of the distribution of specific mRNAs in the polysomal and free mRNP populations of both cytoplasmic fractions revealed differences in the pattern of their distribution. It is further demonstrated that in the L6 rat myoblast system, ribosomes and mRNA (or mRNP) are not associated with the microfilaments, unlike in other systems studied.

摘要

在分化的L6大鼠成肌细胞中研究了细胞骨架在蛋白质合成中的重要性。对成肌细胞和肌管进行温和的非离子去污剂裂解后获得的可溶性和细胞骨架组分，分析其中核糖体和mRNA-蛋白质复合物（mRNPs）的存在情况。多聚核糖体mRNPs主要与细胞骨架框架相关，而游离mRNPs则存在于可溶性和细胞骨架组分中。对两个细胞质组分的多聚核糖体和游离mRNP群体中特定mRNA分布的检查揭示了它们分布模式的差异。进一步证明，在L6大鼠成肌细胞系统中，与其他研究的系统不同，核糖体和mRNA（或mRNP）不与微丝相关。