Charbord P, Tippens D, Wight T S, Gown A M, Singer J W
Exp Hematol. 1987 Jan;15(1):72-7.
This report describes an IgG1 mouse monoclonal antibody derived after immunization of mice with washed stromal cells from human, long-term bone marrow cultures. The antigen recognized by the antibody (BMS-1) is a carbohydrate-containing prosthetic group that is common to and specific for multiple horse serum proteins. These proteins are avidly ingested by stromal cells and concentrated in endocytic vesicles. Cultured smooth muscle cells took up the horse proteins in a similar manner to marrow stromal cells while cultured marrow fibroblasts, endothelial cells, and hepatoma cells did not. These data indicate that marrow stromal cells specifically accumulate horse serum proteins which might partially explain the horse serum requirement for long-term marrow culture maintenance. The data also suggest further similarities between marrow stromal and smooth muscle cells and additional differences between marrow fibroblasts and marrow stromal cells.
本报告描述了一种IgG1小鼠单克隆抗体，它是在用来自人长期骨髓培养物的洗涤基质细胞免疫小鼠后获得的。该抗体（BMS-1）识别的抗原是一种含碳水化合物的辅基，它是多种马血清蛋白所共有的且具有特异性。这些蛋白质被基质细胞 avidly摄取并浓缩在内吞小泡中。培养的平滑肌细胞以与骨髓基质细胞相似的方式摄取马蛋白，而培养的骨髓成纤维细胞、内皮细胞和肝癌细胞则不摄取。这些数据表明骨髓基质细胞特异性积累马血清蛋白，这可能部分解释了长期骨髓培养维持对马血清的需求。这些数据还表明骨髓基质细胞和平滑肌细胞之间存在进一步的相似性，以及骨髓成纤维细胞和骨髓基质细胞之间存在更多差异。
