Charbord P, Lerat H, Newton I, Tamayo E, Gown A M, Singer J W, Herve P
Blood Bank and Bone Marrow Transplantation Unit, Besançon, France.
Exp Hematol. 1990 May;18(4):276-82.
The cytoskeleton of stromal cells from the adherent layer of human Dexter-type cultures has been studied. It was found that the stress fibers contained actin specific for smooth muscle, mainly the alpha SM actin isoform. The intermediate filaments consisted of vimentin, and there were no desmin filaments. This pattern was similar to that of cultured vascular smooth muscle cells. The detectability of the alpha SM actin isoform is coincident with the appearance of stromal cells in long-term marrow cultures and may provide a useful marker for stromal cells. The potential in vivo cellular counterpart for stromal cells generated in the Dexter-type culture system is discussed.
对人德克斯特型培养物贴壁层基质细胞的细胞骨架进行了研究。发现应力纤维含有平滑肌特异性肌动蛋白，主要是α-SM肌动蛋白异构体。中间丝由波形蛋白组成，不存在结蛋白丝。这种模式与培养的血管平滑肌细胞相似。α-SM肌动蛋白异构体的可检测性与长期骨髓培养中基质细胞的出现一致，可能为基质细胞提供一个有用的标志物。讨论了在德克斯特型培养系统中产生的基质细胞在体内潜在的细胞对应物。
