Donnér J, Eriksson H, Belfrage P
FEBS Lett. 1986 Nov 24;208(2):269-72. doi: 10.1016/0014-5793(86)81030-4.
The acute anti-lipolytic effect of human growth hormone (hGH) in maximally noradrenaline-stimulated intact rat adipocytes was selectively associated with increased phosphorylation of a 46 kDa plasma membrane protein which was highly enriched by hGH-Sepharose chromatography. The same protein was also phosphorylated by an endogenous protein kinase in isolated plasma membranes, although then no hGH effect could be demonstrated. About 14% of the phosphate incorporated into the protein in isolated plasma membranes was found in tyrosine residues and the remainder in serine and threonine. The possible relation of the 46 kDa protein with the hGH plasma membrane receptor is discussed.
人生长激素（hGH）对去甲肾上腺素最大刺激的完整大鼠脂肪细胞的急性抗脂解作用，与一种46 kDa质膜蛋白磷酸化增加选择性相关，该蛋白通过hGH-琼脂糖凝胶层析高度富集。相同的蛋白在分离的质膜中也被一种内源性蛋白激酶磷酸化，不过那时未显示出hGH效应。在分离的质膜中，掺入该蛋白的磷酸盐约14%存在于酪氨酸残基中，其余存在于丝氨酸和苏氨酸中。讨论了46 kDa蛋白与hGH质膜受体的可能关系。
