Hashimoto S, Nakajima R, Yamazaki I, Tatsuno Y, Kitagawa T
FEBS Lett. 1986 Nov 24;208(2):305-7. doi: 10.1016/0014-5793(86)81038-9.
Resonance Raman spectra were observed for compound II of horseradish peroxidase A2, and the Fe(IV) = O stretching Raman line was identified at 775 cm-1. This Raman line shifted to 741 cm-1 upon a change of solvent from H2(16)O to H2(18)O, indicating occurrence of the oxygen exchange between the Fe(IV) = O heme and bulk water. The oxygen exchange took place only at the acidic side of the heme-linked ionization with pKa = 6.9.
观察到辣根过氧化物酶A2的化合物II的共振拉曼光谱，并在775cm-1处鉴定出Fe(IV)=O伸缩拉曼谱线。当溶剂从H2(16)O变为H2(18)O时，该拉曼谱线移至741cm-1，表明Fe(IV)=O血红素与大量水之间发生了氧交换。氧交换仅在血红素连接的电离的酸性侧发生，pKa = 6.9。
