Gou Yuanyuan, Wang Zichen, Zhou Liping, Du Jinpan, Huang Jiaxin, Li Jing, Zhang Xuyu, Guan Su
MOE Joint International Research Laboratory of Synthetic Biology and Medicine, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, PR China.
Evaluation and Monitoring Center of Occupational Health, Guangzhou Twelfth People's Hospital, Guangzhou, 510620, PR China.
Heliyon. 2023 Sep 16;9(9):e20162. doi: 10.1016/j.heliyon.2023.e20162. eCollection 2023 Sep.
Inflammatory bowel disease is a relapsing inflammatory disease seriously endanger human health. Wedelolactone (WED) is a major active ingredient from and has shown anti-inflammatory effects. However, the mechanism of WED in treating inflammatory colitis remains unknown. We aimed to investigate the mechanisms of WED in treating ulcerative colitis through lipidomic study. Sixty male C57BL/6 mice were exposed to DSS to induce acute colitis. Disease progression was judged by the disease activity index (DAI) and pathological changes of colon tissue. An ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) method was performed for colon and plasma lipidomics analyses. Differential metabolites in the three groups were distinguished by univariate and multivariate analysis. WED exerted anti-inflammatory effects representing by body weight and DAI score. Three metabolites were identified in plasma and 20 in colon. According to pathway analysis, the effects of WED on colitis were associated with seven pathways. The glycerophospholipid metabolism and ether lipid metabolism were the primary pathways. The findings provide important insight of the mechanism of WED in treating DSS induced colitis through lipidomic perspective.
炎症性肠病是一种严重危害人类健康的复发性炎症性疾病。水甘草内酯（WED）是[植物名称未给出]的主要活性成分，已显示出抗炎作用。然而，WED治疗炎症性结肠炎的机制尚不清楚。我们旨在通过脂质组学研究探讨WED治疗溃疡性结肠炎的机制。将60只雄性C57BL/6小鼠暴露于葡聚糖硫酸钠（DSS）以诱导急性结肠炎。通过疾病活动指数（DAI）和结肠组织的病理变化判断疾病进展。采用超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-TOF-MS）方法对结肠和血浆进行脂质组学分析。通过单变量和多变量分析区分三组中的差异代谢物。WED以体重和DAI评分表示发挥抗炎作用。在血浆中鉴定出3种代谢物，在结肠中鉴定出20种。根据通路分析，WED对结肠炎的作用与7条通路相关。甘油磷脂代谢和醚脂代谢是主要通路。这些发现从脂质组学角度为WED治疗DSS诱导的结肠炎的机制提供了重要见解。
