长链非编码RNA SUMO1P3通过miR-486-5p/PHF8/CD151加重食管鳞状细胞癌细胞的恶性行为。

LncSUMO1P3 exacerbates malignant behaviors of esophageal squamous cell carcinoma cells via miR-486-5p/PHF8/CD151.

作者信息

Zhang Tingyou, Yue Guojun, Tian Xin, Xu Ying, Li Zhongwen

机构信息

Department of Oncology, The Second Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou, China.

出版信息

Heliyon. 2023 Aug 23;9(9):e19110. doi: 10.1016/j.heliyon.2023.e19110. eCollection 2023 Sep.

DOI:10.1016/j.heliyon.2023.e19110

PMID:37809985

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10558295/

Abstract

BACKGROUND

Esophageal squamous cell carcinoma (ESCC) is a malignancy usually associated with smoking or alcohol consumption. The involvement of long noncoding RNAs (lncRNAs) in the regulation of tumor development and metastasis through molecular mechanisms has been unveiled by accumulating evidence. However, the function of lncRNA SUMO1 Pseudogene 3 (lncSUMO1P3) essential to ESCC development remains obscure.

METHODS

Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot (WB) analysis were done to measure RNA and protein levels. Functional assays were carried out to examine the changes in ESCC cell phenotype. Supported by bioinformatics analysis, mechanism assays were done for assessment of putative interactions among different genes.

RESULTS

LlncSUMO1P3 was aberrantly up-regulated in ESCC cell lines, and lncSUMO1P3 deficiency could hamper cell proliferation, migration and invasion as well as epithelial-mesenchymaltransition (EMT) in ESCC while lncSUMO1P3 overexpression led to the opposite consequences. LncSUMO1P3 could competitively bind to microRNA-486-5p (miR-486-5p) or PHD finger protein 8 (PHF8) to modulate CD151 expression. CD151 was also verified to regulate ESCC cell biological behaviors.

CONCLUSION

Our study revealed that lncSUMO1P3, up-regulated in ESCC cells, could sponge miR-486-5p and recruit PHF8 to up-regulate CD151, thus influencing the malignant behaviors of ESCC cells.

摘要

背景

食管鳞状细胞癌（ESCC）是一种通常与吸烟或饮酒相关的恶性肿瘤。越来越多的证据揭示了长链非编码RNA（lncRNAs）通过分子机制参与肿瘤发生发展和转移的调控。然而，lncRNA SUMO1假基因3（lncSUMO1P3）在ESCC发生发展中的重要作用仍不清楚。

方法

采用逆转录定量实时聚合酶链反应（RT-qPCR）和蛋白质免疫印迹（WB）分析来检测RNA和蛋白质水平。进行功能实验以检测ESCC细胞表型的变化。在生物信息学分析的支持下，进行机制实验以评估不同基因之间的潜在相互作用。

结果

lncSUMO1P3在ESCC细胞系中异常上调，lncSUMO1P3缺失会阻碍ESCC细胞的增殖、迁移、侵袭以及上皮-间质转化（EMT），而lncSUMO1P3过表达则会导致相反的结果。lncSUMO1P3可以竞争性结合微小RNA-486-5p（miR-486-5p）或PHD指蛋白8（PHF8）来调节CD151的表达。CD151也被证实可调节ESCC细胞的生物学行为。