工程化人类心脏类器官的方案综述。
A review of protocols for engineering human cardiac organoids.
作者信息
Wu Fujian, He Qian, Li Furong, Yang Xiaofei
机构信息
Translational Medicine Collaborative Innovation Center, Shenzhen People's Hospital (The First Affiliated Hospital, Southern University of Science and Technology, The Second Clinical Medical College of Jinan University), Shenzhen, 518055, Guangdong, China.
Post-doctoral Scientific Research Station of Basic Medicine, Jinan University, Guangzhou, 510632, China.
出版信息
Heliyon. 2023 Sep 7;9(9):e19938. doi: 10.1016/j.heliyon.2023.e19938. eCollection 2023 Sep.
The use of human cardiac organoids (hCOs) as 3D in vitro models for cardiovascular research has shown great promise. Human pluripotent stem cells (hPSCs) have proven to be a potent source for engineering hCOs. However, various protocols for generating hCOs from hPSCs result in significant differences in heart development, maturity, complexity, vascularization, and spatial structure, all of which can influence their functional and physiological properties. This protocol review aims to highlight different strategies for generating hCOs using hPSCs while also critically discussing their challenges and limitations.
将人类心脏类器官（hCOs）用作心血管研究的三维体外模型已显示出巨大的前景。人类多能干细胞（hPSCs）已被证明是构建hCOs的有效来源。然而，从hPSCs生成hCOs的各种方案在心脏发育、成熟度、复杂性、血管化和空间结构方面存在显著差异，所有这些都会影响它们的功能和生理特性。本方案综述旨在突出使用hPSCs生成hCOs的不同策略，同时批判性地讨论它们面临的挑战和局限性。
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