Chen Fan, Ma Yizhe, Cui Ying, Wang Wanhang, Mei Chenchen, Nie Jingjing, Wen Congcong, Shen Xiuwei, Zhou Xuzhao
Ruian People's Hospital, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, China.
Int J Anal Chem. 2023 Sep 28;2023:4747771. doi: 10.1155/2023/4747771. eCollection 2023.
An ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of tenacissoside G, tenacissoside H, and tenacissoside I in rat plasma. The rat plasma was treated with liquid-liquid extraction using ethyl acetate. The determination was performed on the UPLC HSS T3 column (50 mm × 2.1 mm, 1.8 m) with a mobile phase consisting of acetonitrile-water (containing 0.1% formic acid) and gradient elution at a flow rate of 0.4 mL/min. Electrospray (ESI) positive ion mode detection and multireaction monitoring (MRM) quantitative analysis were performed. A total of 36 rats were given tenacissoside G, tenacissoside H, and tenacissoside I, respectively, orally (5 mg/kg) and intravenously (1 mg/kg), with 6 rats in each group, to evaluate the pharmacokinetic difference of tenacissoside G, tenacissoside H, and tenacissoside I in rats. The calibration curves showed good linearity in the range of 5-2000 ng/mL, where was greater than 0.99. The results of precision, accuracy, recovery, matrix effect, and stability met the requirements of biological sample detection methods. The established UPLC-MS/MS method was successfully applied to pharmacokinetic studies of tenacissoside G, tenacissoside H, and tenacissoside I, and the bioavailability was 22.9%, 89.8%, and 9.4%, respectively.
建立了一种超高效液相色谱-串联质谱（UPLC-MS/MS）法测定大鼠血浆中藤糖苷G、藤糖苷H和藤糖苷I。大鼠血浆采用乙酸乙酯液-液萃取法处理。测定在UPLC HSS T3柱（50 mm×2.1 mm，1.8 μm）上进行，流动相为乙腈-水（含0.1%甲酸），梯度洗脱，流速为0.4 mL/min。采用电喷雾（ESI）正离子模式检测和多反应监测（MRM）定量分析。将36只大鼠分别口服（5 mg/kg）和静脉注射（1 mg/kg）藤糖苷G、藤糖苷H和藤糖苷I，每组6只，以评估藤糖苷G、藤糖苷H和藤糖苷I在大鼠体内的药代动力学差异。校准曲线在5-2000 ng/mL范围内呈良好线性，相关系数大于0.99。精密度、准确度、回收率、基质效应和稳定性结果均符合生物样品检测方法的要求。所建立的UPLC-MS/MS方法成功应用于藤糖苷G、藤糖苷H和藤糖苷I的药代动力学研究，生物利用度分别为22.9%、89.8%和9.4%。
