Bolton J P, Takahashi A, Kawauchi H, Kubota J, Hirano T
Gen Comp Endocrinol. 1986 May;62(2):230-8. doi: 10.1016/0016-6480(86)90113-9.
A highly specific and sensitive radioimmunoassay (RIA) for the measurement of plasma and pituitary growth hormone (GH) levels in salmonid fishes was developed using an anti-serum raised in rabbit against chum salmon (Oncorhynchus keta) GH (sGH). Pituitary extracts and plasma from chum, coho, masu, and amago salmon, and from rainbow trout and Japanese charr, all exhibited displacement curves parallel to the sGH standard. Samples from the eel, carp, goldfish, and tilapia, as well as plasma from hypophysectomized chum salmon and rainbow trout, all showed negligible cross-reactivity. None of the mammalian or teleostean GH or prolactin preparations tested cross-reacted with the antibody in the assay system. RIA sensitivity was 0.6 ng sGH/ml of plasma when 100 microliter of plasma was employed. Intra- and interassay coefficients of variation were 3.9 and 4.1%, respectively. Plasma GH levels of the mature chum salmon caught in Otsuchi Bay were highly variable, especially in females (20.2 +/- 8.2 ng/ml) as compared with males (16.0 +/- 1.1 ng/ml), and there was no significant change after transfer to fresh water. Whereas there was no change in plasma GH levels in males kept in seawater, the levels in females increased with time in close correlation with the increase in plasma chloride.
利用兔抗鲑鱼生长激素（sGH）的抗血清，开发了一种用于测定鲑科鱼类血浆和垂体生长激素（GH）水平的高特异性和灵敏的放射免疫分析（RIA）方法。来自狗鲑、银鲑、马苏大麻哈鱼、秋鲑，以及虹鳟和日本红点鲑的垂体提取物和血浆，均呈现出与sGH标准品平行的置换曲线。来自鳗鱼、鲤鱼、金鱼和罗非鱼的样本，以及垂体切除的狗鲑和虹鳟的血浆，交叉反应均可忽略不计。所测试的哺乳动物或硬骨鱼类的GH或催乳素制剂在该分析系统中均未与抗体发生交叉反应。当使用100微升血浆时，RIA的灵敏度为0.6纳克sGH/毫升血浆。批内和批间变异系数分别为3.9%和4.1%。在大槌湾捕获的成熟狗鲑的血浆GH水平高度可变，尤其是雌性（20.2±8.2纳克/毫升），相比之下雄性（16.0±1.1纳克/毫升），转移到淡水中后没有显著变化。雄性海水饲养的狗鲑血浆GH水平没有变化，而雌性狗鲑的血浆GH水平随时间增加，与血浆氯化物的增加密切相关。
