Qin Yifei, Shi Zheng, Zhu Longji, Li Hongzhe, Lu Wenjia, Ye Guozhu, Huang Qiansheng, Cui Li
Xiamen Key Laboratory of Indoor Air and Health, Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China.
Environ Sci Technol. 2023 Oct 24;57(42):15858-15868. doi: 10.1021/acs.est.3c04800. Epub 2023 Oct 9.
Long-term exposure to the indoor environment may pose threats to human health due to the presence of pathogenic bacteria and their byproducts. Nanoscale extracellular vesicles (EVs) extensively secreted from pathogenic bacteria can traverse biological barriers and affect physio-pathological processes. However, the potential health impact of EVs from indoor dust and the underlying mechanisms remain largely unexplored. Here, Raman spectroscopy combined with multiomics (genomics and proteomics) was used to address these issues. Genomic analysis revealed that was an efficient producer of EVs that harbored 68 types of virulence factor-encoding genes. Upon exposing macrophages to environmentally relevant doses of PAO1-derived EVs, macrophage internalization was observed, and release of inflammatory factors was determined by RT-PCR. Subsequent Raman spectroscopy and unsupervised surprisal analysis of EV-affected macrophages distinguished metabolic alterations, particularly in proteins and lipids. Proteomic analysis further revealed differential expression of proteins in inflammatory and metabolism-related pathways, indicating that EV exposure induced macrophage metabolic reprogramming and inflammation. Collectively, our findings revealed that pathogen-derived EVs in the indoor environments can act as a new mediator for pathogens to exert adverse health effects. Our method of Raman integrated with multiomics offers a complementary approach for rapid and in-depth understanding of EVs' impact.
由于致病细菌及其副产物的存在，长期暴露于室内环境可能对人类健康构成威胁。致病细菌广泛分泌的纳米级细胞外囊泡（EVs）可以穿越生物屏障并影响生理病理过程。然而，室内灰尘中EVs对健康的潜在影响及其潜在机制在很大程度上仍未得到探索。在此，利用拉曼光谱结合多组学（基因组学和蛋白质组学）来解决这些问题。基因组分析表明， 是EVs的高效生产者，其携带68种毒力因子编码基因。将巨噬细胞暴露于环境相关剂量的源自PAO1的EVs后，观察到巨噬细胞内化，并通过RT-PCR测定炎症因子的释放。随后，对受EV影响的巨噬细胞进行拉曼光谱和无监督意外分析，区分了代谢变化，特别是蛋白质和脂质方面的变化。蛋白质组学分析进一步揭示了炎症和代谢相关途径中蛋白质的差异表达，表明EV暴露诱导了巨噬细胞代谢重编程和炎症。总体而言,我们的研究结果表明，室内环境中病原体衍生的EVs可作为病原体产生不良健康影响的新介质。我们的拉曼与多组学相结合的方法为快速深入了解EVs的影响提供了一种补充方法。
