Hartland B J, Timoney J F
Appl Environ Microbiol. 1979 Mar;37(3):517-20. doi: 10.1128/aem.37.3.517-520.1979.
American oysters, Crassostrea virginica, and hard clams, Mercenaria mercenaria, were experimentally contaminated with Escherichia coli, Salmonella typhimurium, and Shigella flexneri either by intracardial injection or via the natural route of ingestion. Bacterial inactivation in the hemolymph was monitored for 72 h after exposure to these enteric pathogens at 20 and 6 degrees C. At 6 degrees C, both mean bacterial uptake by ingestion and subsequent clearance was singificantly lower that at 20 degrees C. However, substantial bacterial clearance from the hemolymph occurred for both shellfish at each temperature. At 20 degrees C, viable bacteria were no longer detectable after 24 h in hemolymph of either clams or oysters after exposure to contaminated water containing 4 x 10(3) bacteria per ml.
美国牡蛎（Crassostrea virginica）和硬壳蛤（Mercenaria mercenaria）通过心内注射或经自然摄食途径，被实验性地感染了大肠杆菌、鼠伤寒沙门氏菌和弗氏志贺氏菌。在20摄氏度和6摄氏度下，将这些贝类暴露于这些肠道病原体后，监测血淋巴中细菌的失活情况，持续72小时。在6摄氏度时，通过摄食摄入的细菌平均量及其后续清除率均显著低于20摄氏度时的情况。然而，在每个温度下，两种贝类的血淋巴中细菌都有大量清除。在20摄氏度时，将蛤和牡蛎暴露于每毫升含有4×10³个细菌的污染水中后，24小时后在其血淋巴中就不再能检测到活细菌。
