Timoney J F, Abston A
Appl Environ Microbiol. 1984 May;47(5):986-8. doi: 10.1128/aem.47.5.986-988.1984.
A simple, in vitro protocol was devised to study contamination by and subsequent elimination of Escherichia coli and Salmonella typhimurium in the hard clam, Mercenaria mercenaria. The test bacteria were eliminated rapidly at similar rates for 8 h after exposure and less rapidly thereafter. At 24 h, numbers of E. coli had declined more than S. typhimurium. Bacteria were cleared in the form of rapidly sedimenting fecal and pseudofecal particulates with which the bacteria were stably associated. Ionic bonding was apparently not involved in this association. Degradation of substantial numbers of bacteria occurred in feces at between 6 and 24 h.
设计了一种简单的体外实验方案,以研究硬壳蛤(Mercenaria mercenaria)中大肠杆菌和鼠伤寒沙门氏菌的污染情况以及随后的清除情况。暴露后8小时内,受试细菌以相似的速率迅速被清除,之后清除速率降低。在24小时时,大肠杆菌数量的下降幅度大于鼠伤寒沙门氏菌。细菌以快速沉降的粪便和假粪便颗粒的形式被清除,这些颗粒与细菌稳定结合。这种结合显然不涉及离子键。在6至24小时之间,粪便中有大量细菌发生降解。