Camper A K, McFeters G A
Appl Environ Microbiol. 1979 Mar;37(3):633-41. doi: 10.1128/aem.37.3.633-641.1979.
Injury induced in Escherichia coli cells by chlorination was studied from a physiological standpoint. Predictable and reproducible injury was found to occur rapidly in 0.5 mg of chlorine per liter and was reversible under nonselective conditions. There was an extended lag period in the growth of chlorinated cells not seen in control suspensions followed by the resumption of logarithmic growth at a rate equaling that of control cells. The aldolase activity of cells chlorinated in vivo was equivalent to that obtained for control cells. Oxygen uptake experiments showed that chlorinated cells underwent a decrease in respiration that was not immediatedly repaired in the presence of reducing agents. This effect was more pronouned in rich media containing reducing agents. Uptake of metabolities was inhibited by chlorine injury as shown with experiments using 14C-labeled glucose and algal protein hydrolysate.
从生理学角度研究了氯化作用对大肠杆菌细胞造成的损伤。发现每升0.5毫克氯会迅速导致可预测且可重复的损伤，并且在非选择性条件下这种损伤是可逆的。氯化细胞的生长存在一个比对照悬浮液中更长的延迟期，随后以与对照细胞相同的速率恢复对数生长。体内氯化细胞的醛缩酶活性与对照细胞相当。氧气摄取实验表明，氯化细胞的呼吸作用减弱，在还原剂存在的情况下这种减弱不会立即得到修复。在含有还原剂的丰富培养基中这种效应更为明显。如使用14C标记的葡萄糖和藻类蛋白水解物进行的实验所示，代谢物的摄取受到氯损伤的抑制。
