Dockins W S, McFeters G A
Appl Environ Microbiol. 1978 Aug;36(2):341-8. doi: 10.1128/aem.36.2.341-348.1978.
The physiological basis of the Eijkman elevated-temperature test for differentiating fecal from nonfecal coliforms was investigated. Manometric studies indicated that the inhibitory effect upon growth and metabolism in a nonfecal coliform at 44.5 degrees C involved cellular components common to both aerobic and fermentative metabolism of lactose. Radioactive substrate incorporation experiments implicated cell membrane function as a principal focus for temperature sensitivity at 44.5 degrees C. A temperature increase from 35 to 44.5 degrees C drastically reduced the rates of [14C]glucose uptake in nonfecal coliforms, whereas those of fecal coliforms were essentially unchanged. In addition, relatively low levels of nonfecal coliform beta-galactosidase activity coupled with thermal inactivation of this enzyme at a comparatively low temperature may also inhibit growth and metabolism of nonfecal coliforms at the elevated temperature.
对艾克曼高温试验用于区分粪大肠菌群和非粪大肠菌群的生理基础进行了研究。压力测定研究表明，在44.5摄氏度时对非粪大肠菌群生长和代谢的抑制作用涉及乳糖需氧代谢和发酵代谢共有的细胞成分。放射性底物掺入实验表明细胞膜功能是44.5摄氏度时温度敏感性的主要焦点。温度从35摄氏度升高到44.5摄氏度会大幅降低非粪大肠菌群对[14C]葡萄糖的摄取速率，而粪大肠菌群的摄取速率基本不变。此外，非粪大肠菌群β-半乳糖苷酶活性相对较低，再加上该酶在相对较低温度下的热失活，也可能在高温下抑制非粪大肠菌群的生长和代谢。
