Biology and Health Laboratory, UFR des Biosciences, Félix Houphouët-Boigny University, 22 BP 582, Abidjan 22, Côte d'Ivoire.
Department of Biochemistry-Genetics, UFR o f Biological Sciences, Péléforo Gon Coulibaly University, BP 1328, Korhogo, Côte d'Ivoire.
Chem Biodivers. 2023 Nov;20(11):e202301010. doi: 10.1002/cbdv.202301010. Epub 2023 Nov 5.
The objective of this study was to study the diversity of endophytic fungi isolated from Ceiba pentandra and to isolate their bioactive chemical compounds. The methodology used during this study consisted in isolating endophytic fungi from the bark of C. pentandra on Potato Agar. The isolates obtained were identified on the basis of the ITS regions of their ribosomal DNA. Antibacterial screening of the mycelium of endophytic fungi isolated was evaluated against multidrug-resistant E. coli and S. aureus strains. This screening led to the selection of isolates EC 321 and EC 28 for their ability to effectively inhibit the growth of the bacterial strains tested. EC 321 was grown and fermented on rice medium. Secondary metabolites were extracted with ethyl acetate. From the crude extract, secalonic acid A was isolated and identified by chromatographic and NMR. The in vitro activity of secalonic acid A against the growth of multiresistant bacterial strains was evaluated. Secalonic acid A was active against all multidrug-resistant bacterial strains E. coli 942, E. coli 4814, S. aureus 931, S. aureus 934, S. aureus MRSA 1872 and K. pneumonia 815 with respective MICs of 18.75; 18.75; 18.75; 4.7; 37.5 and 37.5 μg/mL.
本研究旨在研究从 Ceiba pentandra 中分离出的内生真菌的多样性，并分离其生物活性化学化合物。本研究中使用的方法包括在马铃薯琼脂上从 C. pentandra 的树皮中分离内生真菌。获得的分离物基于其核糖体 DNA 的 ITS 区域进行鉴定。对分离出的内生真菌的菌丝进行了针对多药耐药性大肠杆菌和金黄色葡萄球菌的抗菌筛选。该筛选导致选择 EC 321 和 EC 28 分离物，因为它们能够有效抑制测试的细菌菌株的生长。EC 321 在大米培养基上生长和发酵。用乙酸乙酯提取次生代谢产物。从粗提物中分离出并通过色谱和 NMR 鉴定了 secalonic 酸 A。评估了 secalonic 酸 A 对多药耐药细菌菌株生长的体外活性。secalonic 酸 A 对所有多药耐药的大肠杆菌菌株 942、大肠杆菌 4814、金黄色葡萄球菌 931、金黄色葡萄球菌 934、耐甲氧西林金黄色葡萄球菌 1872 和肺炎克雷伯菌 815 均具有活性，MIC 分别为 18.75；18.75；18.75；4.7；37.5 和 37.5 μg/mL。
