Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, School of Tropical Medicine, Hainan Medical University , Haikou, Hainan, China.
Nanobiosensing and Microfluidic Point-of-Care Testing Key Laboratory of LuZhou , Luzhou, Sichuan, China.
Microbiol Spectr. 2023 Dec 12;11(6):e0112523. doi: 10.1128/spectrum.01125-23. Epub 2023 Oct 10.
This study focused on the development of a reaction system using rhPCR to amplify a specific gene, ORF2, of and to identify the P174L mutation associated with increased drug resistance to ceftazidime (CAZ). The system incorporated universal primer probes and a simple temperature cycle reaction. The amplified products were then analyzed using lateral flow strip assay (LFSA) for strain identification and mutation interpretation. The developed system provides a reliable basis for diagnosing melioidosis and selecting appropriate drugs. Its potential impact is particularly significant in resource-limited settings where access to advanced diagnostic techniques is limited. This platform stands out for its simplicity, convenience, sensitivity, specificity, and portability. It shows promise as a point-of-care testing method for detecting single nucleotide polymorphism in genes associated with other diseases. By leveraging the advantages of this platform, researchers and healthcare professionals can potentially expand its use beyond melioidosis and apply it to the rapid detection of genetic variations in other disease-related genes.
本研究旨在开发一种使用 rhPCR 扩增 特定基因 ORF2 的反应系统，并鉴定与头孢他啶（CAZ）耐药性增加相关的 P174L 突变。该系统结合了通用引物探针和简单的温度循环反应。然后使用侧向流动条检测（LFSA）分析扩增产物，以进行菌株鉴定和突变解释。所开发的系统为诊断类鼻疽病和选择合适的药物提供了可靠的依据。在资源有限的环境中，由于无法获得先进的诊断技术，该系统的潜在影响尤其重要。该平台具有简单、方便、灵敏、特异和便携等特点。它有望成为一种用于检测与其他疾病相关基因中单核苷酸多态性的即时检测方法。通过利用该平台的优势，研究人员和医疗保健专业人员可以将其应用范围从类鼻疽病扩展到其他与疾病相关基因的遗传变异的快速检测。
