苦杏仁甙（扁桃苷）——存在于杏仁核中的维生素 B17 对大白鼠受照射唾液腺的影响。

Effect of vitamin B17 (amygdalin) found in apricot kernel on the irradiated salivary glands of albino rats.

机构信息

Department of Oral Biology, Faculty of Oral and Dental Medicine, Cairo University, Egypt.

出版信息

Dent Med Probl. 2023 Jul-Sep;60(3):473-481. doi: 10.17219/dmp/132387.

DOI:10.17219/dmp/132387

PMID:37815512

Abstract

BACKGROUND

Radiotherapy is used as a treatment for head and neck cancers but increases the risk of salivary gland hypofunction. The management strategies include pharmacotherapies such as salivary substitutes and sialagogues which are largely temporary. In this study, we examine the regenerative potential of vitamin B17 to improve salivary gland function.

OBJECTIVES

The present investigation aims to identify the effect of vitamin B17 (amygdaline) on the irradiated parotid salivary gland of albino rats.

MATERIAL AND METHODS

Twenty-eight adult male albino rats were randomly divided into two groups subjected to irradiation procedure. Fourteen were in the control group, receiving a daily 5 mL saline by oral gavage (7 rats for 14 days and 7 rats for 30 days) while the other fourteen were treated with a daily dose of vitamin B17 (grounded apricot kernel; GAK) at 400 mg/kg in 5 mL of saline by oral gavage (7 rats for 14 days and 7 rats for 30 days). The parotid glands were dissected from the two groups at 14 and 30 days from the day of exposure to irradiation. The parotid gland sections were subjected to H&E stain, immunohistochemical localization of epidermal growth factor (EGF) and PCR using transforming growth factor beta 2 (TGF-β2).

RESULTS

The histological abnormalities corroborate with the immunohistochemical localization of EGF and the PCR results of TGF β2, as their up-regulation in the control group demonstrate oxidative stresses and inflammation. The Treatment with GAK decreased oxidative stress and inflammation while promoting tissue regeneration.

CONCLUSIONS

Vitamin B17 is a promising anti-inflammatory agent that boosts immunity, as the experimental group showed better histological architecture of the parotid gland than the other one.

摘要

背景

放射疗法被用于治疗头颈部癌症，但会增加唾液腺功能低下的风险。管理策略包括使用唾液替代物和唾液刺激剂等药物治疗，但这些方法在很大程度上是暂时的。在这项研究中，我们研究了维生素 B17 改善唾液腺功能的再生潜力。

目的

本研究旨在确定维生素 B17（苦杏仁苷）对大白鼠放射性腮腺的影响。

材料和方法

将 28 只成年雄性大白鼠随机分为两组，进行放射处理。其中 14 只作为对照组，每天经口灌胃 5 mL 生理盐水（7 只用于 14 天，7 只用于 30 天），另 14 只给予 400mg/kg 的维生素 B17（粉碎杏仁核；GAK），每天经口灌胃 5 mL（7 只用于 14 天，7 只用于 30 天）。从暴露于放射的第一天起，14 天和 30 天后，从两组中取出腮腺。对腮腺切片进行 H&E 染色、表皮生长因子（EGF）的免疫组织化学定位和转化生长因子β2（TGF-β2）的 PCR。