Ramos Flávia Maria de Moraes, Pontual Maria Luiza Dos Anjos, de Almeida Solange Maria, Bóscolo Frab Norberto, Tabchoury Cinthia Pereira Machado, Novaes Pedro Duarte
Oral Radiology, School of Dentistry of Piracicaba, University of Campinas, Piracicaba, São Paulo, SP, Brazil.
Arch Oral Biol. 2006 Feb;51(2):96-101. doi: 10.1016/j.archoralbio.2005.06.008. Epub 2005 Aug 8.
The aim of this study was to evaluate the radioprotective effect of vitamin E in salivary gland function, as well as analyse the total protein concentration. For this purpose 90 male rats were used and randomly divided into five experimental groups: control (I), in which animals received olive oil solution but were not irradiated; irradiated-olive oil (II), in which animals received olive oil solution and were irradiated with a single exposure dose of 15 Gy of gamma rays to the head and neck region; irradiated (III), in which animals were only irradiated with a single exposure dose of 15 Gy of gamma rays; vitamin E (IV), in which animals received alpha tocopherol acetate solution but were not irradiated; irradiated-vitamin E (V), in which animals received alpha tocopherol acetate solution before irradiation with a single exposure dose of 15 Gy gamma rays. The animals were sacrificed 4, 8 h and 30 days after the irradiation procedure. No differences were observed in salivary volumes between the groups at 4 and 8 h. At 30 days, the salivary volume in the animals pertaining to the irradiated-olive oil group was significantly reduced in relation to the control group. The only irradiated group (III) presented significantly diminished salivary volume. In the salivary composition, no significant differences were observed in the total protein content between the groups studied. It was concluded that radiation had no effect on the total protein content and that vitamin E protected the salivary function 30 days after irradiation. Thus, vitamin E can be considered as a potential radioprotective substance.
本研究的目的是评估维生素E对唾液腺功能的辐射防护作用，并分析总蛋白浓度。为此，使用了90只雄性大鼠，并将其随机分为五个实验组：对照组（I），动物接受橄榄油溶液但未接受照射；照射-橄榄油组（II），动物接受橄榄油溶液并对头颈部区域进行单次15 Gy的γ射线照射；照射组（III），动物仅接受单次15 Gy的γ射线照射；维生素E组（IV），动物接受醋酸α-生育酚溶液但未接受照射；照射-维生素E组（V），动物在接受单次15 Gyγ射线照射前接受醋酸α-生育酚溶液。在照射程序后4、8小时和30天处死动物。在4小时和8小时时，各组之间的唾液量未观察到差异。在30天时，照射-橄榄油组动物的唾液量相对于对照组显著减少。仅照射组（III）的唾液量显著减少。在唾液成分方面，所研究的各组之间总蛋白含量未观察到显著差异。得出的结论是，辐射对总蛋白含量没有影响，并且维生素E在照射后30天保护了唾液功能。因此，维生素E可被视为一种潜在的辐射防护物质。
