Han Dongwei, Zhang Di, Han Dezhi, Ren Honglei, Wang Zhen, Zhu Zhijia, Sun Haoyue, Wang Lianxia, Qu Zhongcheng, Lu Wencheng, Yuan Ming
Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences, Qiqihar, China.
Heihe Branch of Heilongjiang Academy of Agricultural Sciences, Heihe, China.
Sci Rep. 2023 Oct 10;13(1):17057. doi: 10.1038/s41598-023-44266-5.
Salt is recognized as one of the most major factors that limits soybean yield in acidic soils. Soil enzyme activity and bacterial community have a critical function in improving the tolerance to soybean. Our aim was to assess the activities of soil enzyme, the structure of bacteria and their potential functions for salt resistance between Salt-tolerant (Salt-T) and -sensitive (Salt-S) soybean genotypes when subject to salt stress. Plant biomass, soil physicochemical properties, soil catalase, urease, sucrase, amylase, and acid phosphatase activities, and rhizosphere microbial characteristics were investigated in Salt-T and Salt-S soybean genotypes under salt stress with a pot experiment. Salt stress significantly decreased the soil enzyme activities and changed the rhizosphere microbial structure in a genotype-dependent manner. In addition, 46 ASVs which were enriched in the Salt-T geotype under the salt stress, such as ASV19 (Alicyclobacillus), ASV132 (Tumebacillus), ASV1760 (Mycobacterium) and ASV1357 (Bacillus), which may enhance the tolerance to soybean under salt stress. Moreover, the network structure of Salt-T soybean was simplified by salt stress, which may result in soil bacterial communities being susceptible to external factors. Salt stress altered the strength of soil enzyme activities and the assembly of microbial structure in Salt-T and Salt-S soybean genotypes. Na, NO-N, NH-N and Olsen-P were the most important driving factors in the structure of bacterial community in both genotypes. Salt-T genotypes enriched several microorganisms that contributed to enhance salt tolerance in soybeans, such as Alicyclobacillus, Tumebacillus, and Bacillus. Nevertheless, the simplified network structure of salt-T genotype due to salt stress may render its bacterial community structure unstable and susceptible.
盐被认为是限制酸性土壤中大豆产量的最主要因素之一。土壤酶活性和细菌群落对提高大豆耐受性具有关键作用。我们的目的是评估耐盐（Salt-T）和盐敏感（Salt-S）大豆基因型在盐胁迫下土壤酶活性、细菌结构及其抗盐潜在功能。通过盆栽试验研究了盐胁迫下Salt-T和Salt-S大豆基因型的植物生物量、土壤理化性质、土壤过氧化氢酶、脲酶、蔗糖酶、淀粉酶和酸性磷酸酶活性以及根际微生物特征。盐胁迫显著降低了土壤酶活性，并以基因型依赖的方式改变了根际微生物结构。此外，在盐胁迫下，Salt-T基因型中富集了46个ASV，如ASV19（ Alicyclobacillus）、ASV132（Tumebacillus）、ASV1760（Mycobacterium）和ASV1357（Bacillus），这些可能增强大豆在盐胁迫下的耐受性。此外，盐胁迫简化了Salt-T大豆的网络结构，这可能导致土壤细菌群落易受外部因素影响。盐胁迫改变了Salt-T和Salt-S大豆基因型中土壤酶活性强度和微生物结构组装。Na、NO-N、NH-N和Olsen-P是两种基因型细菌群落结构中最重要的驱动因素。Salt-T基因型富集了几种有助于提高大豆耐盐性的微生物，如 Alicyclobacillus、Tumebacillus和Bacillus。然而，盐胁迫导致的Salt-T基因型网络结构简化可能使其细菌群落结构不稳定且易受影响。
