Kishen Akansha, Cecil Anju, Chitra S
Saveetha Dental College and hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai-600077, Tamil Nadu, India.
Department of Periodontics, Saveetha Dental College and hospitals Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai-600077, Tamil Nadu, India.
Saudi Dent J. 2023 Sep;35(6):678-683. doi: 10.1016/j.sdentj.2023.05.021. Epub 2023 May 30.
The regeneration of lost/damaged support tissue in the periodontium, including the alveolar bone, periodontal ligament, and cementum, is an ambitious purpose of periodontal regenerative therapy and might effectively reduce periodontitis-caused tooth loss. Guided tissue regeneration (GTR) is a technique currently used in dentistry for periodontal surgery, which allows osseous regeneration prior to soft tissue migration into the area of interest. Calcium phosphate-based bone grafts (mostly Tricalcium Phosphate or Hydroxyapatite) are bio ceramics that show the greatest similarity to the mineral found in the bone. Thereby, giving calcium-phosphate excellent biocompatibility, biodegradability and osteoconductivity. The aim of the study is to fabricate hydroxyapatite reinforced polymeric hydrogel membrane for regeneration.
Pure alginate fabrication was done by cross linking sodium alginate with calcium chloride. Hydroxyapatite (HAP) alginate (Alg) was formulated by adding nanoparticles to the alginate mixture, which was then cross-linked with calcium chloride to formulate a HAP alginate polymeric membrane. The Fourier-transform infrared spectroscopy (FT-IR), Scanning Electron Microscope (SEM), and biocompatibility tests were performed to analyse the membrane characteristics.
Fabricated Hydroxyapatite- alginate (Hap- Alg) membrane has longer durability, because of strong crystal structure which in turn might take a longer time to regenerate. The membrane was found to be biocompatible and HAp induces faster mineralisation which in turn will increase the tissue regeneration rate of the membrane.
The findings of our study suggests that the HAP-Alg hydro gel membrane is highly durable and hemocompatible and it has faster mineralisation capability thus making it superior from the clinically available membranes for GTR. Further analyses needs to be conducted to evaluate the potential of this membrane to be used for regeneration.
牙周组织中包括牙槽骨、牙周膜和牙骨质在内的缺失/受损支持组织的再生，是牙周再生治疗的一个宏伟目标，并且可能有效减少牙周炎导致的牙齿脱落。引导组织再生（GTR）是目前牙科用于牙周手术的一种技术，它能使骨再生先于软组织迁移至感兴趣区域。磷酸钙基骨移植材料（主要是磷酸三钙或羟基磷灰石）是与骨中发现的矿物质最相似的生物陶瓷。因此，赋予了磷酸钙优异的生物相容性、生物降解性和骨传导性。本研究的目的是制备用于再生的羟基磷灰石增强聚合物水凝胶膜。
通过将海藻酸钠与氯化钙交联来制备纯海藻酸盐。通过向海藻酸盐混合物中添加纳米颗粒来配制羟基磷灰石（HAP）海藻酸盐（Alg），然后将其与氯化钙交联以配制HAP海藻酸盐聚合物膜。进行傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）和生物相容性测试以分析膜的特性。
制备的羟基磷灰石-海藻酸盐（Hap-Alg）膜具有更长的耐久性，因为其晶体结构坚固，这反过来可能需要更长时间才能再生。该膜被发现具有生物相容性，并且HAp能诱导更快的矿化，这反过来将提高膜的组织再生率。
我们的研究结果表明，HAP-Alg水凝胶膜具有高度耐久性和血液相容性，并且具有更快的矿化能力，因此使其优于临床上可用于GTR的膜。需要进行进一步分析以评估该膜用于再生的潜力。
