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用于牙周再生的四角葡萄藤掺杂细胞外基质和含透明质酸支架的开发：一项体外研究。

Development of a Cissus quadrangularis-Doped Extracellular Matrix and a Hyaluronic Acid-Incorporated Scaffold for Periodontal Regeneration: An In Vitro Study.

作者信息

Ganesh S Balaji, Sabu Abraham, Kaarthikeyan G, Eswaramoorthy Rajalakshmanan, P T Priyangha

机构信息

Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.

Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.

出版信息

Cureus. 2024 Mar 19;16(3):e56507. doi: 10.7759/cureus.56507. eCollection 2024 Mar.

DOI:10.7759/cureus.56507
PMID:38646344
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11026305/
Abstract

PURPOSE

The study aimed to analyze whether adding (CQ) extract and the extracellular matrix of ovine tendon (TENDON) increases the regenerative potential of mesenchymal stem cells produced in hyaluronic acid (HA) scaffolds for tenogenesis.

MATERIALS AND METHODS

Fifty grams of powdered CQ was mixed with 250 mL of ethanol to prepare the extract. Two grams of hyaluronic acid powder was added to 100 mL of distilled water to make the HA solution. The ovine tendon was decellularized using a mixture of 10% phosphate-buffered saline (PBS), sodium dodecyl sulfate (SDS), and Triton-X. The hydrogel samples were prepared by mixing the extracellular matrix of tendon, HA, and CQ, after which they were divided into study groups such as HA, HA + CQ, HA + TENDON, and HA + CQ + TENDON. Scanning electron microscopy (SEM) analysis, swelling analysis, differentiation analysis, compression test, compatibility assay, and tenogenesis assay were later conducted.

RESULTS

The morphology of the samples was analyzed using SEM. Low levels of swelling of the hydrogels were observed. Cells were found to be viable and showed good differentiation and tenogenesis. Optimal compression levels were observed, and the properties of the prepared hydrogels were satisfactory.

CONCLUSION

The results suggest that the addition of CQ considerably increases the tenogenic potential of the extracellular matrix/HA scaffold. Hence, it can be used as a regenerative material for periodontal tissue regeneration.

摘要

目的

本研究旨在分析添加（CQ）提取物和羊肌腱细胞外基质（TENDON）是否能提高在透明质酸（HA）支架中产生的间充质干细胞用于肌腱生成的再生潜力。

材料与方法

将50克CQ粉末与250毫升乙醇混合制备提取物。将2克透明质酸粉末加入100毫升蒸馏水中制成HA溶液。使用10%磷酸盐缓冲盐水（PBS）、十二烷基硫酸钠（SDS）和曲拉通-X的混合物对羊肌腱进行脱细胞处理。通过混合肌腱细胞外基质、HA和CQ制备水凝胶样品，然后将其分为HA、HA + CQ、HA + TENDON和HA + CQ + TENDON等研究组。随后进行扫描电子显微镜（SEM）分析、肿胀分析、分化分析、压缩试验、相容性测定和肌腱生成测定。

结果

使用SEM分析样品的形态。观察到水凝胶的肿胀程度较低。发现细胞具有活力，并表现出良好的分化和肌腱生成能力。观察到最佳压缩水平，制备的水凝胶性能令人满意。

结论

结果表明，添加CQ可显著提高细胞外基质/HA支架的肌腱生成潜力。因此，它可作为牙周组织再生的再生材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/2d5e63167d2a/cureus-0016-00000056507-i11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/60a4acfc0365/cureus-0016-00000056507-i01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/e10e0eb91747/cureus-0016-00000056507-i02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/aeea2f237368/cureus-0016-00000056507-i03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/17dadbbf99da/cureus-0016-00000056507-i04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/11161c35a60d/cureus-0016-00000056507-i05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/5d4c1a71b876/cureus-0016-00000056507-i06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/6559bed1bd76/cureus-0016-00000056507-i07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/7f3e89fb14ac/cureus-0016-00000056507-i08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/4b71ff4df2a9/cureus-0016-00000056507-i09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/1dde10ea0625/cureus-0016-00000056507-i10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/2d5e63167d2a/cureus-0016-00000056507-i11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/60a4acfc0365/cureus-0016-00000056507-i01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/e10e0eb91747/cureus-0016-00000056507-i02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/aeea2f237368/cureus-0016-00000056507-i03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/17dadbbf99da/cureus-0016-00000056507-i04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/11161c35a60d/cureus-0016-00000056507-i05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/5d4c1a71b876/cureus-0016-00000056507-i06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/6559bed1bd76/cureus-0016-00000056507-i07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/7f3e89fb14ac/cureus-0016-00000056507-i08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/4b71ff4df2a9/cureus-0016-00000056507-i09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/1dde10ea0625/cureus-0016-00000056507-i10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dea2/11026305/2d5e63167d2a/cureus-0016-00000056507-i11.jpg

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